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>   home   >   Products   >   Primary Antibodies   >   Anti Rat MHC Class II RT1Bu/L Antibody, clone OX-3    

Anti Rat MHC Class II RT1Bu/L Antibody, clone OX-3

Mouse Anti-Rat Monoclonal Antibody

     
  • IHC - Anti Rat MHC Class II RT1Bu/L Antibody, clone OX-3  ABD12536
    Published customer image:Mouse anti Rat MHC class II antibody, clone OX-3 used to label MHC class II expressing cells in formalin fixed mouse brain cryosections by immunohistochemistry.Image caption:(a) Representative immunostained micrographs show Iba1+ cells in the SN of 3-, 6-, 9-, and 12-month-old female and male mice. Scale bar: 10?μm. Quantitative results of Iba1+ cell areas (b) and the number of Iba1+ cells (c) in the SN of female and male mice at different ages (n?=?5). *(p <0.05), **(p <0.01), ***(p <0.001): versus the opposite sex. (d) Representative immunostained micrographs show MHC class II+ cells in the SN of 3-, 6-, 9-, and 12-month-old female and male mice. Scale bar: 10?μm. Quantitative results of MHC class II+ cell areas (e) and the number of MHC class II+ cells (f) in the SN of female and male mice at different ages (n?=?5). ***(p <0.001): versus the opposite sex. (g) Representative immunostained micrographs show TH+ cells in the SN of 3-, 6-, 9-, and 12-month-old female and male mice. Scale bar: 200?μm. (h) Quantitative results of TH+ cells in the SN of female and male mice at different ages (n?=?5). *(p <0.05), **(p <0.01): versus the opposite sex.From: Wu SY, Chen YW, Tsai SF, Wu SN, Shih YH, Jiang-Shieh YF, Yang TT, Kuo YM.Estrogen ameliorates microglial activation by inhibiting the Kir2.1 inward-rectifier K(+) channel.Sci Rep. 2016 Mar 10;6:22864.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Reactivity Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names OX-3
Additional Information
Other Species M
Purification Purified IgG prepared by ion exchange chromatography
Immunogen Rat thymocyte membrane glycoproteins.
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti Rat MHC Class II RT1Bu/L antibody, clone OX-3 recognizes a polymorphic determinant of the rat RT1B MHC class II antigen, reacting with haplotypes u and l. The literature reports reactivity with Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains. This antibody is useful for distinguishing RT1B positive cells from different rat strains, e.g. for recognising cells of donor origin in bone marrow reconstituted radiation chimaeras.The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region.Mouse anti Rat MHC Class II RT1Bu/L antibody, clone OX-3 also cross reacts with mouse strains of the H-2 haplotypes b and s. Analysis of recombinant mouse strains has mapped the OX-3 determinant to the H-2I-A region. This product is routinely tested in flow cytometry on Lewis rat splenocytes.
Preservative & Stabilisers 0.09% Sodium Azide, 1% Bovine Serum Albumin
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Rat MHC Class II RT1Bu/L Antibody, clone OX-3 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12536
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