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>   home   >   Products   >   Primary Antibodies   >   Cardiovascular   >   Anti Human CD73 Antibody, clone 2B6    

Anti Human CD73 Antibody, clone 2B6

Mouse Anti-Human Monoclonal Antibody

  • WB - Anti Human CD73 Antibody, clone 2B6  ABD12547
    Western blot analysis of CD73 expression on human mesenchymal stem cell lysates using Mouse anti Human CD73, clone 2B6
  • IF - Anti Human CD73 Antibody, clone 2B6  ABD12547
    Published customer image:Mouse anti Human CD73 antibody, clone 2B6 used for the detection of CD73 fusion proteins in transfected COS-7 cells by imunofluorescence and western blotting.Image caption: Characterization of DsRed-NT5E fusion proteins. A. COS-7 cells were transiently transfected with an expression vector for DsRed fused to wild type form of human NT5E (DsRed-hNT5E WT) and fusion proteins were localized by immunofluorescence with antibodies against fluorescent DsRed and native hNT5E proteins, and by internal fluorescence . The merged picture shows obvious overlap of all 3 signals indicating that DsRed-hNT5E fusion protein is intact and contains both antigens. Magnification 400X. B. Immunoblot analysis of total protein extracts from mock-transfected or transfected COS-7 cells with expression vectors for DsRed monomer and DsRed-hNT5E (DsRed NT5E) fusion protein using DsRed and hNT5E antibodies. Both antibodies detect a protein with a molecular weight of approximately 90 kiloDaltons (black arrowheads, left and right) while DsRed antibody only detects the DsRed monomer (white arrowhead, left) indicating that DsRed-hNT5E fusion is intact and contains both antigens. Total β-actin protein was used as loading control. C. Enzyme histochemistry of mock-transfected or transfected COS-7 cells with expression vectors for DsRed-hNT5E wild type and mutant (F1–3) proteins using adenosine monophosphate as substrate. Only COS–7 cells transfected with the expression vector for DsRed-hNT5E wild type fusion protein displayed AMPase activity, which could be blocked in presence of known NT5E inhibitor α/β-methylene-ADP. Mock-transfected or COS-7 cells transfected with expression vectors for DsRed-hNT5E mutant (F1–3) proteins lack AMPase activity. Magnification 200X.From: Fausther M, Lavoie EG, Goree JR, Baldini G, Dranoff JA NT5E Mutations That Cause Human Disease Are Associated with Intracellular Mistrafficking of NT5E Protein.PLoS ONE 9(6): e98568.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P21589
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Clone Names 2B6
Calculated MW 63368 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Recombinant human CD73 (amino acids 27-252) purified from E. coli.
Shelf Life 18 months from date of despatch.
Gene ID 4907
Other Names 5'-nucleotidase, 5'-NT,, Ecto-5'-nucleotidase, CD73, NT5E, NT5, NTE
Target/Specificity Mouse anti Human CD73, clone 2B6 recognises human CD73, otherwise known as ecto-5'-nucleotidase, a GPI-anchored enzyme and member of the 5'-nucleotidase family, which catalyzes the dephosphorylation of 5'-mononucleotides into their corresponding nucleosides, and can also mediate co-stimulatory signals in T cell activation (Dianzaniet al.1993).CD73 is expressed by subpopulations of T and B lymphocytes, and increases during lymphocyte development, acting as a lymphocyte maturation marker. A decrease in CD73 activity has been associated with several immunodeficiency disorders including AIDS and Wiskott-Aldrich syndrome, and CD73 is expressed on a variety of lymphomas and leukaemias, including CLL and ALL (Zhaoet al.2011)
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human CD73 Antibody, clone 2B6 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12547
(40 western blots)
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