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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti Mouse CD4 Antibody, clone GK1.5    

Anti Mouse CD4 Antibody, clone GK1.5

Rat Anti-Mouse Monoclonal Antibody

     
  • FC - Anti Mouse CD4 Antibody, clone GK1.5  ABD12556
    Staining of mouse spleen with Rat anti Mouse CD4
  • FC - Anti Mouse CD4 Antibody, clone GK1.5  ABD12556
    Published customer image:Intrafollicular location of MZB cells in B6.TC spleens. A. Representative spleen sections from 3 mo old B6.TC and B6 mice stained with Moma-1-FITC, CD1d-PE and B220-PB. The B220+ CD1d+ MZB cells show as bright pink while the other B cells show as blue. The ring of Moma-1+ metallophillic macrophages delineates the MZ inner edge. B. Percentage of CD1d+ B220+ B cells relative to total B220+ B cells outside and inside the Moma-1+ ring in 3 mo and 10 mo old B6 and B6.TC mice. The data show means and standard errors of the mean calculated of 4 MZ and FO areas for each mouse. ***: p < 0.001 for t tests. C. Representative spleen sections from 10 mo old B6.TC and B6 mice stained with CD4-FITC, CD1d-PE and B220-PB. Boxed areas show multiple contacts between green B6.TC CD4 T cells and pink MZB cells, but not in the B6 spleen. Original magnification: 200X.From:Zhou et al. BMC Immunology 2011 12:7
  • IHC - Anti Mouse CD4 Antibody, clone GK1.5  ABD12556
    Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD4 antibody, clone GK1.5 followed by horseradish peroxidase conjugated Goat anti Rat IgG . Medium power
  • IHC - Anti Mouse CD4 Antibody, clone GK1.5  ABD12556
    Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD4 antibody, clone GK1.5 followed by horseradish peroxidase conjugated Goat anti Rat IgG . High power
  • IF - Anti Mouse CD4 Antibody, clone GK1.5  ABD12556
    Published customer image:Rat anti Mouse CD4 antibody, clone GK1.5 used for the identification of CD4 expressing cells in tumors by immunofluorescence.Image caption:LAG-3 and CD4 expression in the tumor microenvironment after MVA-BN-HER2 and anti-PD-1 therapy. (A) Tumors from mice treated with MVA-BN-HER2 and/or anti-PD-1 were collected on day 16 and stained for LAG-3 , CD4 , and nuclei (DAPI, blue). (B) The area density for CD8+ T cells.* p<0.05. n = 3–4 mice/group.From: Foy SP, Sennino B, dela Cruz T, Cote JJ, Gordon EJ, Kemp F, et al.Poxvirus-Based Active Immunotherapy with PD-1 and LAG-3 Dual Immune Checkpoint Inhibition Overcomes Compensatory Immune Regulation, Yielding Complete Tumor Regression in Mice.PLoS ONE 11(2): e0150084.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-F, IF, FC, E, IP
Primary Accession P06332
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2b
Clone Names GK1.5
Calculated MW 51297 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Murine CD4.
Shelf Life 18 months from the date of despatch
Gene ID 12504
Other Names T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, CD4, Cd4
Target/Specificity Rat anti Mouse CD4 antibody, clone GK1.5 recognizes mouse CD4, a ~55 kDa protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on T helper cells, peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+CD25+), which play a key role in the maintenance of self tolerance. Rat anti Mouse CD4 antibody, clone GK1.5 has been reported to block CD4+ T-cell activation. It blocks class II MHC antigen-specific binding, thereby inhibiting functions such as class II MHC antigen-specific proliferation and the release of lymphokines. It may also be used for in vivoandin vitro cell depletion of CD4+T-cells.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4oC or at -20oC if preferred.
PrecautionsAnti Mouse CD4 Antibody, clone GK1.5 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12556
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