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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti Mouse CD4 Antibody, clone GK1.5    

Anti Mouse CD4 Antibody, clone GK1.5

Rat Anti-Mouse Monoclonal Antibody

  • FC - Anti Mouse CD4 Antibody, clone GK1.5  ABD12556
    Staining of mouse spleen with Rat anti Mouse CD4
  • FC - Anti Mouse CD4 Antibody, clone GK1.5  ABD12556
    Published customer image:Intrafollicular location of MZB cells in B6.TC spleens. A. Representative spleen sections from 3 mo old B6.TC and B6 mice stained with Moma-1-FITC, CD1d-PE and B220-PB. The B220+ CD1d+ MZB cells show as bright pink while the other B cells show as blue. The ring of Moma-1+ metallophillic macrophages delineates the MZ inner edge. B. Percentage of CD1d+ B220+ B cells relative to total B220+ B cells outside and inside the Moma-1+ ring in 3 mo and 10 mo old B6 and B6.TC mice. The data show means and standard errors of the mean calculated of 4 MZ and FO areas for each mouse. ***: p < 0.001 for t tests. C. Representative spleen sections from 10 mo old B6.TC and B6 mice stained with CD4-FITC, CD1d-PE and B220-PB. Boxed areas show multiple contacts between green B6.TC CD4 T cells and pink MZB cells, but not in the B6 spleen. Original magnification: 200X.From:Zhou et al. BMC Immunology 2011 12:7
  • IHC - Anti Mouse CD4 Antibody, clone GK1.5  ABD12556
    Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD4 antibody, clone GK1.5 followed by horseradish peroxidase conjugated Goat anti Rat IgG . Medium power
  • IHC - Anti Mouse CD4 Antibody, clone GK1.5  ABD12556
    Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD4 antibody, clone GK1.5 followed by horseradish peroxidase conjugated Goat anti Rat IgG . High power
  • IF - Anti Mouse CD4 Antibody, clone GK1.5  ABD12556
    Published customer image:Rat anti Mouse CD4 antibody, clone GK1.5 used for the identification of CD4 expressing cells in tumors by immunofluorescence.Image caption:LAG-3 and CD4 expression in the tumor microenvironment after MVA-BN-HER2 and anti-PD-1 therapy. (A) Tumors from mice treated with MVA-BN-HER2 and/or anti-PD-1 were collected on day 16 and stained for LAG-3 , CD4 , and nuclei (DAPI, blue). (B) The area density for CD8+ T cells.* p<0.05. n = 3–4 mice/group.From: Foy SP, Sennino B, dela Cruz T, Cote JJ, Gordon EJ, Kemp F, et al.Poxvirus-Based Active Immunotherapy with PD-1 and LAG-3 Dual Immune Checkpoint Inhibition Overcomes Compensatory Immune Regulation, Yielding Complete Tumor Regression in Mice.PLoS ONE 11(2): e0150084.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P06332
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2b
Clone Names GK1.5
Calculated MW 51297 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Murine CD4.
Shelf Life 18 months from the date of despatch
Gene ID 12504
Other Names T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, CD4, Cd4
Target/Specificity Rat anti Mouse CD4 antibody, clone GK1.5 recognizes mouse CD4, a ~55 kDa protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on T helper cells, peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+CD25+), which play a key role in the maintenance of self tolerance. Rat anti Mouse CD4 antibody, clone GK1.5 has been reported to block CD4+ T-cell activation. It blocks class II MHC antigen-specific binding, thereby inhibiting functions such as class II MHC antigen-specific proliferation and the release of lymphokines. It may also be used for in vivoandin vitro cell depletion of CD4+T-cells.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4oC or at -20oC if preferred.
PrecautionsAnti Mouse CD4 Antibody, clone GK1.5 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12556
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