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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti Human CD3 Antibody, clone UCHT1    

Anti Human CD3 Antibody, clone UCHT1

Mouse Anti-Human Monoclonal Antibody

     
  • WB - Anti Human CD3 Antibody, clone UCHT1  ABD12559
    Published customer image:FITC conjugated Mouse anti Human CD3 antibody, clone UCHT1 used for the evaluation of CD3 expression on human PBMC by flow cytometry.Image caption:Expression of RANTES in obese subjects. (a) Western blot analysis of RANTES expression in PBMCs from lean and obese subjects. The bands reacting with anti-RANTES antibody were quantified as described in Section 2 and the relative intensity was determined after correction with Actin that was used as internal control to monitor loading efficiency. The blots shown are representative of three independent experiments with consistent results. The data are presented in the form of a bar graph on the right of the figure as fold changes of RANTES protein expression in obese compared to lean subjects. (b) Characterization of the monocyte subpopulations and T cells in peripheral blood from lean and obese participants. Monocytes subsets were defined by staining for CD14 , T cells by CD3 , and expression of intracellular RANTES was analyzed. Left and right upper panels are representative dot plots of CD3 and RANTES expression on/in T cells from lean and obese participants, respectively. Left and right lowest panels are representative dot plots of CD14 and RANTES expression on/in monocyte subsets from lean and obese participants, respectively. The double-positive populations (i.e., CD3+RANTES+, CD14+RANTES+, and CD14++RANTES+) were analyzed for mean RANTES fluorescence intensity. (c) Analysis of RANTES expression by immunohistochemistry in the subcutaneous adipose tissues from lean and obese nondiabetic participants. Aperio software was used to quantify positive staining (indicated by arrows) and quantified values relative to lean controls are plotted in a bar graph at the bottom. (d and e) Analysis of RANTES mRNA expression by quantitative real-time PCR between lean and obese subjects. Total RNA was isolated from PBMCs (d) and adipose tissue biopsies (e). The data are presented as fold changes in obese compared to lean subjects after normalization with the GAPDH reference gene. (f and g) Analysis of TNF-a and IL-6 expression at the protein level by IHC (f) and at the mRNA level by qRT-PCR (g) in the adipose tissue from lean and obese subjects. In IHC experiments, Aperio software was used to quantify positive staining as indicated above and the values are illustrated at the bottom as fold changes compared to lean. Mann-Whitney test was used to determine significance of difference between the lean and obese subjects. For each experiment, the sample size from each group is indicated by n.From: Engin Baturcam, Jehad Abubaker, Ali Tiss, et al., “Physical Exercise Reduces the Expression of RANTES and Its CCR5 Receptor in the Adipose Tissue of Obese Humans,”Mediators of Inflammation, vol. 2014, Article ID 627150, 13 pages, 2014.
  • IHC - Anti Human CD3 Antibody, clone UCHT1  ABD12559
    Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. High power
  • FC - Anti Human CD3 Antibody, clone UCHT1  ABD12559
    Published customer image:FITC conjugated Mouse anti Human CD3 antibody, clone UCHT1 used for the evaluation of CD3 expression on human PBMC by flow cytometry.Image caption:Differential regulation of CCR5 in PBMCs and adipose tissue is associated with obesity. CCR5 gene expression was measured by qRT-PCR in PBMCs (a) from lean and obese nondiabetic subjects and in the adipose tissue (b) from lean and obese nondiabetic subjects. The data are presented as fold changes in obese compared to lean subjects after normalization with the GAPDH reference gene. (c) Characterization of the monocyte subpopulations and T cells in peripheral blood from lean and obese subjects. Monocytes subsets were defined by staining for CD14 , T cells by CD3 , and expression of CCR5 and then were analyzed. Gates P4 and P3 define the CD14+ and CD14++ subsets, respectively. Left and right upper panels are representative dot plots of CD3 and CCR5 expression on T cells from lean and obese subjects, respectively. Left and right lower panels are representative dot plots of CD14 and RANTES expression on monocyte subsets from lean and obese participants, respectively. The double-positive populations (i.e., CD3+CCR5+, CD14+CCR5+, and CD14++CCR5+) were analyzed for mean CCR5 fluorescence intensity. For each experiment, the sample size from each group is indicated by n.From: Engin Baturcam, Jehad Abubaker, Ali Tiss, et al., “Physical Exercise Reduces the Expression of RANTES and Its CCR5 Receptor in the Adipose Tissue of Obese Humans,”Mediators of Inflammation, vol. 2014, Article ID 627150, 13 pages, 2014.
  • IHC - Anti Human CD3 Antibody, clone UCHT1  ABD12559
    Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. High power
  • E - Anti Human CD3 Antibody, clone UCHT1  ABD12559
    ELISA analysis of human CD3 expression using Rat anti Human CD3ε, clone CD3-12 as a capture reagent and biotinylated Mouse anti Human CD3, clone UCHT1 as a detection reagent with recombinant human CD3 as antigen to produce the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader . A serum sample is included undiluted
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Primary Accession P07766
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names UCHT1
Calculated MW 23147 Da
Additional Information
Other Species Chimpanzee
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome.
Shelf Life 18 months from date of despatch.
Gene ID 916
Other Names T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E
Target/Specificity Mouse anti Human CD3 antibody, clone UCHT1 recognizes the human T-cell surface glycoprotein CD3 epsilon chain, also known as T-cell surface antigen T3/Leu-4 epsilon chain or CD3ε. CD3ε is a 207 amino acid, ~21kDa single pass type 1 transmembrane protein containing a single Ig-like and a single ITAM domain. Mouse anti Human CD3 antibody, clone UCHT1 was originally described as only binding to CD3ε when complexed with either the CD3δ or CD3γ subunits, as indicated by co-transfection immunofluorescence studies on COS cells (Salmerónet al.1991). Mouse anti Human CD3 antibody, clone UCHT1 binds to a region in the ectodomain of human CD3ε and has been shown to bind to a discontinuous epitope near an acidic region of CD3ε opposite the dimer interface; as shown by crystallographic studies of the CD3ε/δ dimer complexed with a single chain UCHT1 antibody fragment (Arnettet al.2004).CD3 is expressed by all T lymphocytes and is seen in all lymphoid organs including lymph nodes and spleen. It is involved in thymocyte differentiation (Brodeuret al.2009). Deficiency of the CD3ε chain contriutes to blocking T-cell development and presentation of a severe combined immunodeficiency phenotype (Fischeret al.2005).Mouse anti Human CD3 antibody, clone UCHT1 has been used successfully for the activation of human paripheral blood lymphocytes by cross linking and subsequently for CD3ε surface espression by flow cytometry (Hirsh and Cohen 2006).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3),Sucrose
Storage Store at +4℃. DO NOT FREEZE.
PrecautionsAnti Human CD3 Antibody, clone UCHT1 is for research use only and not for use in diagnostic or therapeutic procedures.
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