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Anti Human Adiponectin Antibody, clone Adn27

Mouse Anti-Human Monoclonal Antibody

  • E - Anti Human Adiponectin Antibody, clone Adn27  ABD12563
    Sandwich ELISA analysis of human adiponectin binding using Mouse anti Human adiponectin as a capture reagent and biotinylated Mouse anti Human adiponectin as a detection reagent with recombinant human adiponectin as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader . A plasma sample diluted 1:4 is shown
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15848
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names Adn27
Calculated MW 26414 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Recombinant human adiponectin purified from E.coli.
Shelf Life 18 months from date of despatch.
Gene ID 9370
Other Names Adiponectin, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, ACRP30, Adipocyte, C1q and collagen domain-containing protein, Adipose most abundant gene transcript 1 protein, apM-1, Gelatin-binding protein, ADIPOQ, ACDC, ACRP30, APM1, GBP28
Target/Specificity Mouse anti Human Adiponectin antibody, clone Adn27 recognizes human adiponectin, also known as Acrp30, 30 kDa adipocyte complement-related protein, Adipocyte, C1q and collagen domain-containing protei or Gelatin-binding protein. Adiponectin is a 244 amino acid major adipokine secreted into the bloodstream from adipose tissue to modulate metabolism, including glucose regulation and fatty acid catabolism. Unlike most other adipokines, adiponectin is secreted exclusively by differentiating adipocytes at reduced levels during obesity. The serum level of adiponectin is inversely correlated with BMI (body mass index) of an individual, and has an anti-inflammatory action, playing an important role in type II diabetes (insulin sensitivity) and atherosclerosis.Mouse anti human adiponectin antibody, clone Adn27 has been successfully used as a capture reagent with mouse anti human adionectin antibody, clone 5H7 as a detection reagent in a sensitive Sandwich ELISA for the measurement of adiponectin in human plasma.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human Adiponectin Antibody, clone Adn27 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12563
(40 western blots)
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