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Anti Human Adiponectin Antibody, clone Adn36

Mouse Anti-Human Monoclonal Antibody

  • E - Anti Human Adiponectin Antibody, clone Adn36  ABD12564
    Sandwich ELISA analysis of adiponectin binding using Mouse anti Human adiponectin as a capture reagent and biotinylated Mouse anti Human adiponectin as a detection reagent with recombinant human adiponectin as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader . A plasma sample diluted 1:4 is shown
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15848
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names Adn36
Calculated MW 26414 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Recombinant human adiponectin purified from E.coli.
Shelf Life 18 months from date of despatch.
Gene ID 9370
Other Names Adiponectin, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, ACRP30, Adipocyte, C1q and collagen domain-containing protein, Adipose most abundant gene transcript 1 protein, apM-1, Gelatin-binding protein, ADIPOQ, ACDC, ACRP30, APM1, GBP28
Target/Specificity Mouse anti Human Adiponectin antibody, clone Adn36 recognizes human adiponectin, also known as Acrp30, 30 kDa adipocyte complement-related protein, Adipocyte, C1q and collagen domain-containing protein or Gelatin-binding protein. Adiponectin is a 244 amino acid major adipokine secreted into the bloodstream from adipose tissue to modulate metabolism, including glucose regulation and fatty acid catabolism (UniProt: Q15848). Adiponectin circulates as three distinct oligomeric forms, trimers (LMW), hexamers (MMW) and dodecamers-octodecamers (HMW) with distinct functions in various target tissues (Wanget al.2008).Adiponectin is involved in regulation of circulating glucose levels and in regulation of insulin sensitivity. Concentrations of adiponectin are reduced in obesity associated metabolic and vascular disorders (Fasshaueret al.2004) in particular lower circulating levels of HMW adiponectin correleates with the appearance of type II diabetes and acts as a predictor for this condition (Zhuet al.2010).Mouse anti human adiponectin antibody, clone Adn36 has been used successfully in the development of a sensitive sandwich ELISA in conjunction with mouse anti human adiponectin antibody, clone 5H7 as a detection reagent for the measurement of circulating levels of adiponectin in human serum.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human Adiponectin Antibody, clone Adn36 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12564
(40 western blots)
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