|Application ||WB, IHC-P|
|Calculated MW||59216 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||Tuberculin conjugated synthetic peptide LLSHVRHARYAP derived from the C-terminus of human ERB5.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Estrogen receptor beta, ER-beta, Nuclear receptor subfamily 3 group A member 2, ESR2, ESTRB, NR3A2|
|Target/Specificity||Mouse anti human estrogen receptor beta 5, clone 5/25 specifically recognizes human estrogen receptor beta 5 (ERB5), a splice variant of the nuclear receptor hormone ERbeta (ESR2), shown to exist in at least five isoforms (ERB1-ERB5) (Moore et al. 1998). Although only the ERB1 variant appears fully functional, the remaining isoforms have been shown to form heterodimers with ERB1, thereby enhancing transcription (Shaabanet al. 2008).Immunohistochemical studies have revealed a ubiquitous expression of ERB5 in normal colorectal tissue, and to be present in all primary colorectal carcinomas studied (Wong, N. et al. 2005).The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formal fixed paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays (LBA), to become the most common method for the determination of the ER/PR status of breast tumors. Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patient’s prognosis and the effectiveness of hormonal therapy transcription (Shaabanet al. 2008,Collinset al. 2009).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20℃ if preferred.|
|Precautions||Anti Human Estrogen Receptor beta 5 Antibody, clone 5/25 is for research use only and not for use in diagnostic or therapeutic procedures.|
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