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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti Human Estrogen Receptor beta 5 Antibody, clone 5/25    

Anti Human Estrogen Receptor beta 5 Antibody, clone 5/25

Mouse Anti-Human Monoclonal Antibody

     
  • IHC-P - Anti Human Estrogen Receptor beta 5 Antibody, clone 5/25  ABD12583
    Published customer image:Mouse anti Human estrogen receptor β5 antibody, clone 5/25 used for the detrction of ERβ5 expressing cells in normal human liver by immunohistochemistry formalin fixed, paraffin embedded tissue sections.Image caption:Staining pattern of the ERß isoforms in human liver. In order to confirm the specificity of the ERß antibodies used in the study, we immunostained sections of human healthy liver, showing that ERß1 and ERß2 isoforms are not expressed, while specific nuclear ERß5 immunoreactivity is detected (magnification 20x and 40x). This pattern of ERß isoforms immunoreactivity is consistent with previous descriptions.From:Ciucci A, Meco D, De Stefano I, Travaglia D, Zannoni GF, et al. Gender Effect in Experimental Models of Human Medulloblastoma: Does the Estrogen Receptor ß Signaling Play a Role? PLoS ONE 9(7): e101623.
  • IHC-P - Anti Human Estrogen Receptor beta 5 Antibody, clone 5/25  ABD12583
    Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor beta5 antibody followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (high power)
  • IHC - Anti Human Estrogen Receptor beta 5 Antibody, clone 5/25  ABD12583
    Published customer image:Mouse anti Human estrogen receptor β5 antibody, clone 5/25 used for the detection of ERβ5 expressing cells in human endometrial cancer tissue biopsies by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Immunoexpression of ERs in endometrial cancers. Tissues were classified as well (A-D), moderately (E-H) or poorly (J-M) differentiated; main panels show closely adjacent sections from three cancer blocks to allow direct comparisons. All proteins were immunolocalised to cell nuclei (see higher power inserts in panels e, f, c and d respectively). In the well and moderately differentiated cancers expression was most intense in epithelial cell layers (arrowheads, panel A and inserts). Note that ERa was low/absent in poor grade cancers (J) but immunoexpression of ERß1, 2, 5 was readily detected (K. L, M). Inserts in panels K, L, and M show negative controls for ERß1, ERß2 and ERß5 antibodies respectively generated using primary antibodies pre-absorbed with specific peptides used for immunisation. Asterisks (*) label the stromal compartment that was well defined in the well differentiated cancers.From: Collins F, MacPherson S, Brown P, Bombail V, Williams AR, Anderson RA, Jabbour HN, Saunders PT. Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha. BMC Cancer. 2009 Sep 16;9:330.
  • IHC-P - Anti Human Estrogen Receptor beta 5 Antibody, clone 5/25  ABD12583
    Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor beta5 antibody followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (low power)
  • IHC-P - Anti Human Estrogen Receptor beta 5 Antibody, clone 5/25  ABD12583
    Published customer image:Mouse anti Human estrogen receptor β5 antibody, clone 5/25 used for the detrction of ERβ5 expressing cells in tumors by immunohistochemistry formalin fixed, paraffin embedded tissue sections.Image caption:Effect of gender on ERß1, ERß2 and ERß5 expression in D283 Med xenografts. A) Immunostaining for ERß1 was significantly higher in tumors from females compared to males (**p
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q92731
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 5/25
Calculated MW 59216 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Tuberculin conjugated synthetic peptide LLSHVRHARYAP derived from the C-terminus of human ERB5.
Shelf Life 18 months from date of despatch.
Gene ID 2100
Other Names Estrogen receptor beta, ER-beta, Nuclear receptor subfamily 3 group A member 2, ESR2, ESTRB, NR3A2
Target/Specificity Mouse anti human estrogen receptor beta 5, clone 5/25 specifically recognizes human estrogen receptor beta 5 (ERB5), a splice variant of the nuclear receptor hormone ERbeta (ESR2), shown to exist in at least five isoforms (ERB1-ERB5) (Moore et al. 1998). Although only the ERB1 variant appears fully functional, the remaining isoforms have been shown to form heterodimers with ERB1, thereby enhancing transcription (Shaabanet al. 2008).Immunohistochemical studies have revealed a ubiquitous expression of ERB5 in normal colorectal tissue, and to be present in all primary colorectal carcinomas studied (Wong, N. et al. 2005).The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formal fixed paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays (LBA), to become the most common method for the determination of the ER/PR status of breast tumors. Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patient’s prognosis and the effectiveness of hormonal therapy transcription (Shaabanet al. 2008,Collinset al. 2009).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human Estrogen Receptor beta 5 Antibody, clone 5/25 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12583
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