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Anti Human Factor VIII Antibody, clone RFF-VIIIC/8

Mouse Anti-Human Monoclonal Antibody

  • E - Anti Human Factor VIII Antibody, clone RFF-VIIIC/8  ABD12584
    Recombinant Factor VIII detected with Mouse anti Human Factor VIII
  • E - Anti Human Factor VIII Antibody, clone RFF-VIIIC/8  ABD12584
    Published customer image:Insert meta-description and applicationsImage caption:Specificity of FVIII activity assay is demonstrated by antibody inhibition and sample dilutions. (A) FVIII activities, ranging from 0.6–10 mU/ml, in dilutions of rFVIII, were inhibited by addition of 10 μg/ml mouse anti-human FVIII antibody (clone RFF-VIIIC/8) for 10 min (on ice) prior to the start of the chromogenic Coatest assay. The green triangles represent rFVIII without antibody addition and the red squares and blue diamonds are 2 separate rFVIII dilutions with final concentrations of 10 µg/ml anti-FVIII. (B) FVIII activity was measured in HUVEC cell lysates diluted 1.3-, 2-, 3- and 4-fold in 1% BSA/PBS.From: Turner NA, Moake JL Factor VIII Is Synthesized in Human Endothelial Cells, Packaged in Weibel-Palade Bodies and Secreted Bound to ULVWF Strings.PLoS ONE 10: e0140740.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P00451
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names RFF-VIIIC/8
Calculated MW 267009 Da
Additional Information
Other Species Pig
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Affinity purified human Factor VIII.
Shelf Life 18 months from date of despatch.
Gene ID 2157
Other Names Coagulation factor VIII, Antihemophilic factor, AHF, Procoagulant component, Factor VIIIa heavy chain, 200 kDa isoform, Factor VIIIa heavy chain, 92 kDa isoform, Factor VIII B chain, Factor VIIIa light chain, F8, F8C
Target/Specificity Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/8 recognizes human Factor VIII, an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerises and cross-links to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Defects in Factor VIII cause haemophilia A, a disorder characterised by the body’s inability to control blood clotting. This could result in severe blood loss, even with minor injuries. Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/8 is a very potent coagulation inhibitor. It recognizes an epitope towards the N-terminus of full-length Factor VIII. It also recognizes the 210kDa, 90kDa and 40kDa cleavage products. Mouse anti Human Factor VIII antibody, clone RFF-VIIIC/8 does not cross-react with von Willebrand factor.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human Factor VIII Antibody, clone RFF-VIIIC/8 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12584
(40 western blots)
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