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Anti Human von Willebrand Factor Antibody, clone RFF-VIII R/1

Mouse Anti-Human Monoclonal Antibody

  • IHC-P - Anti Human von Willebrand Factor Antibody, clone RFF-VIII R/1  ABD12590
    Staining of paraffin embedded human liver sections with Mouse anti human Von Willebrand Factor
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04275
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names RFF-VIII R/1
Calculated MW 309265 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Human Factor VIII complex partially purified from Factor VIII concentrate.
Shelf Life 18 months from date of despatch.
Gene ID 7450
Other Names von Willebrand factor, vWF, von Willebrand antigen 2, von Willebrand antigen II, VWF, F8VWF
Target/Specificity Mouse anti Human von Willebrand factor antibody, clone RFF-VIII R/1 recognizes human von Willebrand factor (vWF), also known as Factor VIII related antigen, a blood glycoprotein involved in blood coagulation. It stabilises circulating Factor VIII by binding to it and protecting it from cleavage and delivers it to sites of vascular injury. vWF also promotes the adhesion of platelets to sites of vascular damage by forming a molecular bridge between collagen on exposed endothelial cells and the GPIb binding sites of platelets circulating in the blood. vWF circulates in the blood as large multimers, with each monomer (250kDa) containing a number of specific domains. Hereditary or acquired defects in vWF lead to von Willebrand disease (vWD), characterised by varying degrees of susceptibility to bleeding. Symptoms might include nosebleeds, bleeding gums, easy bruising, menorrrhagia or gastrointestinal bleeding. Various forms of vWD exist with differing severities, determined by the type of defect. Mouse anti Human von Willebrand factor, clone RFF-VIII R/1 has a high affinity for an epitope within the platelet GPIb-binding site that is responsible for biological activity. As such the antibody is a potent inhibitor of vWF activity. It can completely neutralise ristocetin-induced platelet aggregation and ristocetin-induced binding of vWF to platelets. It also inhibits platelet adhesion to glass beads. The epitope recognized is present only on the intact multimeric form of vWF and is abolished by mild denaturation with SDS. Mouse anti Human von Willebrand factor, clone RFF-VIII R/1 does not recognize human Factor VIII. Clone RF-VIII R/1 may be used as a capture antibody in immunoassays for vWF in combination with clone RFF-VIII R/2 as a detection reagent.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human von Willebrand Factor Antibody, clone RFF-VIII R/1 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12590
(40 western blots)
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