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Anti Human GAPDH Antibody, clone 4G5

Mouse Anti-Human Monoclonal Antibody

  • WB - Anti Human GAPDH Antibody, clone 4G5  ABD12634
    Western blot analysis of HepG2 human hepatic carcinoma whole cell lysate probed with Mouse anti Human GAPDH antibody followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence
  • WB - Anti Human GAPDH Antibody, clone 4G5  ABD12634
    Published customer image:Mouse anti Human GAPDH antibody, clone 4G5 used as a loading control for the evaluation of rat GAPDH expression by western blotting.Image caption:Dnd1 expression. (A) Huh7 cells were transfected with a cloned GFP-Dnd1 construct and the cDNA was amplified by PCR. This was followed by KpnI restriction digest, to differentiate between the correct wild type and ter Dnd1 cDNA. (B) The GFP-Dnd1 fusion proteins generated in vitro were detectable with an anti GFP and an anti Dnd1 antibody. Both the 66 kDa wild type and the 60 kDa ter GFP-Dnd1 fusion protein could be identified. (C) qRT-PCR. Left: Dnd1 was transcribed in the gonads of animals at 3, 6 and 9 weeks of age. Higher levels were evident in testes compared to ovaries at all ages. Variable amounts were found in teratomas, ranging from high in TGCTs at 3 and 6 weeks, to low in TGCTs at 9 weeks and OGCTs at all ages. Right: Dnd1 expression of three ter/ter animals with unilateral TGCT was measured in both the teratoma and the contralateral degenerated testis. The amount of Dnd1 cDNA found in the teratomas was in the same range as wild type testes, while comparatively low levels were exhibited in the degenerated testis.From: Northrup E, Zschemisch N-H, Eisenblätter R, Glage S, Wedekind D, Cuppen E, et al. The ter Mutation in the Rat Dnd1 Gene Initiates Gonadal Teratomas and Infertility in Both Genders.PLoS ONE 7(5): e38001.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P46406
Other Accession P00355, P16858, P04406, P04797
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 4G5
Calculated MW 35780 Da
Additional Information
Other Species B,Pig,Ga,Cat,Rat,M,Dog,Rb,F
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Human cardiac muscle GADPH.
Shelf Life 18 months from date of despatch.
Gene ID 100009074
Other Names Glyceraldehyde-3-phosphate dehydrogenase, GAPDH,, Peptidyl-cysteine S-nitrosylase GAPDH, 2.6.99.-, GAPDH, GAPD
Target/Specificity Mouse anti Human GAPDH antibody, clone 4G5 recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a 36 kDa protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism. GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death (Chuang & Ishitani 1996). GAPDH binds to several proteins responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin (Burkeet al.1996).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human GAPDH Antibody, clone 4G5 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12634
(40 western blots)
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