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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti Human Talin-2 Antibody, clone 53.8    

Anti Human Talin-2 Antibody, clone 53.8

Mouse Anti-Human Monoclonal Antibody

  • IHC-P - Anti Human Talin-2 Antibody, clone 53.8  ABD12654
    Published customer image:Mouse anti talin-2 antibody, clone 53.8 used for the detection of talin-2 in murine kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Expression of talin1 and talin2 in various mouse tissues and their immuno-localisation in muscle and kidney. (A) Western blot of whole tissue lysates (40 µg protein) probed with the talin1- or talin2-specific antibodies shown. Tissues: B, brain; H, heart; S, spleen; K, kidney; Lu, lung; Li, liver; M, skeletal muscle. (B) Cryo-sections of gastrocnemius muscle were immunostained with Mab 97H6 directed against talin1 (a and b) and Mab 68E7 against talin2 (c–f). Talin1 was mostly localised to the myotendinous junction (arrows in a), with weaker staining of costameres (b), while talin2 staining was strong at both MTJ (arrows in c) and costameres (d). In talin2-null muscle, no staining was detected at the MTJ (arrows in e) or at costameres (f). Scale bar = 50 µm. (C) Immuno-staining of paraffin-embedded sections of wild type and talin2 null mouse kidney with antibodies to talin1 (93E12 and 97H6) and talin2 (68E7 and 121A). Staining is present in the glomerulus, tubules and interstitium in the cortex, and the tubules and interstitium in the medulla. There was no staining for talin2 in kidneys from Tln2-null mice.From: Praekelt U, Kopp PM, Rehm K, Linder S, Bate N, Patel B, Debrand E, Manso AM, Ross RS, Conti F, Zhang MZ, Harris RC, Zent R, Critchley DR, Monkley SJ. New isoform-specific monoclonal antibodies reveal different sub-cellular localisations for talin1 and talin2.Eur J Cell Biol. 2012 Mar;91(3):180-91.
  • IF - Anti Human Talin-2 Antibody, clone 53.8  ABD12654
    Mouse anti Human talin-2 antibody, clone 53.8 used for immunofluorescent staining of isolated talin-1 null cells fixed in paraformaldehyde and permeablized with triton X-100. Originator image
  • WB - Anti Human Talin-2 Antibody, clone 53.8  ABD12654
    Western blotting analysis of anti talin antibody binding to recombinant talin proteins showing specificity of Mouse anti Human talin-2 antibody, clone 53.8 for talin-2, not recognizing recombinant human talin-1. In contrast Mouse anti talin-1 antibody, clone 97H6 does not recognize recombinant human talin-2. Clone 8D4, recognizing both talin isoforms is included.Originator image
  • IF - Anti Human Talin-2 Antibody, clone 53.8  ABD12654
    Mouse anti Human talin-2 antibody, clone 53.8 used for immunofluorescence staining of frozen murine heart sections highlighting both costameres and intercalated discs. Originator image
  • WB - Anti Human Talin-2 Antibody, clone 53.8  ABD12654
    Western blotting analysis of talin-1 and talin-2 expression in murine tissue lysates using Mouse anti Human talin-2 antibody, clone 53.8 and Mouse anti Human talin-1 antibody, clone 97H6 . (B) brain, (H) heart, (S) spleen, (K) kidney, lung, Li , (M) muscle. Originator image
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y4G6
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Clone Names 53.8
Calculated MW 271613 Da
Additional Information
Other Species M
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Recombinant Mouse talin-2 (aa2300-2542).
Shelf Life 18 months from date of despatch.
Gene ID 83660
Other Names Talin-2, TLN2, KIAA0320
Target/Specificity Mouse anti Human Talin-2 antibody, clone 53.8 (also designated 121A) recognizes Talin-2, a high molecular weight skeletal protein involved in focal adhesion plaques. It plays a role in linking integrin to the actin cytoskeleton. It interacts with phosphatidylinositol-4-phosphate 5-kinase type I gamma (PIP5K1C), inducing its kinase activity.Mouse anti Human Talin-2 antibody, clone 53.8 recognizes an epitope within the range of ammino acids 2470-2500.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human Talin-2 Antibody, clone 53.8 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12654
(40 western blots)
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