|Application ||IHC-P, IHC-F, IF, FC, IP|
|Calculated MW||102130 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.|
|Shelf Life||18 months from the date of despatch|
|Other Names||EGF-like module-containing mucin-like hormone receptor-like 1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EMR1 hormone receptor, Emr1, Gpf480|
|Target/Specificity||Rat anti mouse F4/80 antibody, clone Cl:A3-1 recognises the murine F4/80 antigen, a ~160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).Expression of F4/80 is heterogeneous and is modulated during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans cells, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen (Hume,et al.1984). F4/80 antigen is also expressed on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node (Gordon,et al.1994). The ligands and biological functions of the F4/80 antigen have not been fully determined but a role for F4/80 in the generation of efferent CD8+ve regulatory T cells is proposed (Lin,et al.2005)Rat anti mouse F4/80 antibody, clone Cl:A3-1 modulates cytokine levels released in response to Listeria monocytogenes(Warschkau & Kiderlen, 1999).A Human anti-idiotypic CI:A31 antibody, clone 17867 (HCA154 ) which binds to and blocks activity of Rat anti mouse F4/80 antibody, clone Cl:A3-1 is also available for use as a control in experiments utilizing clone A3-1.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20℃ if preferred.|
|Precautions||Anti Mouse F4/80 Antibody, clone Cl:A3-1 is for research use only and not for use in diagnostic or therapeutic procedures.|
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