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Anti Mouse F4/80 Antibody, clone Cl:A3-1

Rat Anti-Mouse Monoclonal Antibody

  • IF - Anti Mouse F4/80 Antibody, clone Cl:A3-1  ABD12667
    Published customer image:Rat anti Mouse F4/80 antibody, clone A3-1 used for the identification of infiltrating macrophages in tumor tissue by immunofluorescence.Image caption:CSF1 has no effect on tumor growth but increases percent tumor TEMs and augments angiogenesis. (A) After two weeks of treatment, tumors were removed, homogenized and immunostained with antibodies specific for F4/80 and Tie2 to identify total F4/80+ cells and F4/80+/Tie2+ cells (Tie2-expressing macrophages, TEMs). While there was a marked increase in total F4/80+ macrophages with CSF1 treatment, the percent of F4/80+/Tie2+ TEMs was significantly increased in response to CSF1 suggesting a regulatory role for CSF1 in expanding the TEM population. N = 5 mice per group and results represent the mean ± SEM of total F4/80+ and F4/80+/Tie2+ TEMs within the tumors. (B, top and bottom left) PyMT tumors without CSF1 treatment and (top and bottom right) with CSF1 treatment immunostained with CD31 for blood vessels, F4/80 for macrophages, Tie2 for F4/80+/Tie2+ TEMS, and DAPI. Confocal images (using 60× objective and with 3× zoom suggest an increase in both F4/80 macrophages and F4/80+/Tie2+ TEMS in the CSF1-treated tumors. Multiply overlap indicates those areas where F4/80 and Tie2 positivity overlap. Individual stains are in Supplementary Figure 3. (C, top) Orthotopically implanted PyMT mammary tumors in wild type C57Bl/6 female mice were allowed to become palpable then intraperitoneally treated with PBS , CSF1 (100 ng in 100 μls) , a neutralizing antibody for the CSF1R (50 mg/kg) 4 hours prior to CSF1 treatment (100 ng in 100 μls) (CSF1R NAb+CSF1), the CSF1R antibody alone (CSF1R NAb), an isotype antibody (50 mg/kg) 4 hours prior to CSF1 (100 ng in 100 μls) treatment (CSF1+IgG), or the isotype antibody alone three times per week for two additional weeks. The tumors were immunostained with a CD31-Alexa Flour 546 antibody to recognize endothelial cells that comprise blood vessels. Qualitatively, CSF1 treatment increased the percent of CD31-postitive pixels per high powered field compared to PBS treated tumors, while the neutralizing antibody to CSF1R suppressed the CSF1 effect on angiogenesis. (B, bottom) Quantitatively, the percent of CD31+ pixels per high powered field were quantified as blood vessels using Adobe Photoshop histogram analysis. CSF1 treatment significantly increased CD31-positive pixels compared to PBS. The neutralizing antibody for CSF1R significantly reduced the ability of CSF1 to up-regulate angiogenesis. N = 5 mice per group and results represent the mean ± SEM of percent CD31-positive pixels per high powered field .From: Forget MA, Voorhees JL, Cole SL, Dakhlallah D, Patterson IL, Gross AC, et al. Macrophage Colony-Stimulating Factor Augments Tie2-Expressing Monocyte Differentiation, Angiogenic Function, and Recruitment in a Mouse Model of Breast Cancer. PLoS ONE 9(6): e98623.
  • IF - Anti Mouse F4/80 Antibody, clone Cl:A3-1  ABD12667
    Published customer image:Rat anti Mouse F4/80 antibody, clone A3-1 used for the detection of microglia in mouse brain by immunofluorescenceImage caption:Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor protein concentrations measured by enzyme-linked immunosorbent assay in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real–time quantitative reverse transcriptase polymerase chain reaction at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN , GFAP , or F4/80 is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI to show all nuclei. Values are mean ± SEM; *P
  • DB - Anti Mouse F4/80 Antibody, clone Cl:A3-1  ABD12667
    Dot Blot showing FSC/SSC gated mouse peritoneal macrophages dual stained with F4/80 at a 1/5 dilution and CD11b at a 1/5 dilution. Isotype control pair in red. Fc receptors were blocked by Mouse Seroblock
  • IF - Anti Mouse F4/80 Antibody, clone Cl:A3-1  ABD12667
    Published customer image:Rat anti Mouse F4/80 antibody, clone A3-1 used for the detection of infiltrating macrophages in tumor tissue by immunofluorescence.Image caption:Presence of inflammatory cells in tumor tissue. Macrophage and neutrophil infiltration are unaffected by loss of serglycin in RIP1-Tag2 tumors. Tumor sections from 15w RTposSGwt and RTposSGko mice were immunostained from the neutrophil and macrophage markers Gr-1 (a) and F4/80 (b) respectively. For Gr-1, the number of positive cells/mm2 was calculated (c) and there was no difference in infiltration of neutrophils between the two groups. For F4/80, the area of positive staining was measured (d) and although there was a slight trend to decreased macrophage infiltration in serglycin deficient animals, this was not significant. Each data point in represents an individual animal. Statistical analysis was performed using a two-tailed Mann-Whitney test. Error bars represent mean ± SEM.From: Hamilton A, Basic V, Andersson S, Abrink M, Ringvall M Loss of Serglycin Promotes Primary Tumor Growth and Vessel Functionality in the RIP1-Tag2 Mouse Model for Spontaneous Insulinoma Formation.PLoS ONE 10(5): e0126688.
  • IHC - Anti Mouse F4/80 Antibody, clone Cl:A3-1  ABD12667
    Published customer image:Rat anti Mouse F4/80 antibody, clone A3-1 used for the detection of infiltrating macrophages in mouse kidney by immunohistochemistry on paraffin embedded materialImage caption:Reduction in cellular infiltration and inflammatory markers in UUO kidneys exposed to telmisartan or PXS64. Untreated UUO kidneys showed increased F4/80, CD68 and CD45 positively stained cells as compared to the sham operated control animals. PXS64 significantly reduced F4/80 and CD45 positive stained cells (Fig. 6C and E) with a trend to a reduction in CD68 cells, although this was not statistically significant (Fig. 6D). Telmisartan treated kidneys showed a reduction in F4/80 positive cells but no difference in CD45 or CD68 stained cells, suggesting a differential action of PXS64 and telmisartan in modifying cellular infiltration. Results are presented as mean showed (n = 8, *P < 0.05 vs. UUO, ** P < 0.01 vs. UUO). Magnification x 400.From: Zhang J, Wong MG, Wong M, Gross S, Chen J, et al. A Cationic-Independent Mannose 6-Phosphate Receptor Inhibitor Ameliorates Kidney Fibrosis by Inhibiting Activation of Transforming Growth Factor-β1.PLoS ONE 10(2): e0116888.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q61549
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2b
Clone Names Cl:A3-1
Calculated MW 102130 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.
Shelf Life 18 months from the date of despatch
Gene ID 13733
Other Names EGF-like module-containing mucin-like hormone receptor-like 1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EMR1 hormone receptor, Emr1, Gpf480
Target/Specificity Rat anti mouse F4/80 antibody, clone Cl:A3-1 recognises the murine F4/80 antigen, a ~160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).Expression of F4/80 is heterogeneous and is modulated during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans cells, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen (Hume,et al.1984). F4/80 antigen is also expressed on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node (Gordon,et al.1994). The ligands and biological functions of the F4/80 antigen have not been fully determined but a role for F4/80 in the generation of efferent CD8+ve regulatory T cells is proposed (Lin,et al.2005)Rat anti mouse F4/80 antibody, clone Cl:A3-1 modulates cytokine levels released in response to Listeria monocytogenes(Warschkau & Kiderlen, 1999).A Human anti-idiotypic CI:A31 antibody, clone 17867 (HCA154 ) which binds to and blocks activity of Rat anti mouse F4/80 antibody, clone Cl:A3-1 is also available for use as a control in experiments utilizing clone A3-1.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Mouse F4/80 Antibody, clone Cl:A3-1 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12667
(40 western blots)
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