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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti Human CD18 Antibody, clone YFC118.3    

Anti Human CD18 Antibody, clone YFC118.3

Rat Anti-Human Monoclonal Antibody

  • FC - Anti Human CD18 Antibody, clone YFC118.3  ABD12671
    Published customer image:R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.
  • FC - Anti Human CD18 Antibody, clone YFC118.3  ABD12671
    Published customer image:Rat anti Human CD18 antibody,clone YFC118.3 used for flow cytometryImage caption: LFA-1 activation analyses by Flow Cytometry. (A) Profiles of CD11a, CD18 activation epitope (CD18act, representing “high affinity” conformation) and CD18 expression by Teff and Treg#1 cells pre-incubated or not with indicated antibodies. Anti-CD28 and anti-CTLA-4 were used at 10 µg/ml. (B) Histograms of Mean Fluorescent Intensity of CD11a, CD18act and CD18tot expressed on Teff and Treg#1 cells. Ratio CD18act MFI: CD18tot was established to analyze LFA-1 high affinity conformation in indicated conditions. Data are representative of more than three different experiments. Filled gray, negative control; Red line, Teff and Treg cells alone; Green line, cells with APC; Blue line, cells with APC and anti-CD28; and Black line: cells with APC, anti-CD28 and anti-CTLA-4.From: Dilek N, Poirier N, Hulin P, Coulon F, Mary C, et al. Targeting CD28, CTLA-4 and PD-L1 Costimulation Differentially Controls Immune Synapses and Function of Human Regulatory and Conventional T-Cells. PLoS ONE 8: e83139.
  • FC - Anti Human CD18 Antibody, clone YFC118.3  ABD12671
    Staining of human peripheral blood granulocytes with Rat anti Human CD18:RPE
  • FC - Anti Human CD18 Antibody, clone YFC118.3  ABD12671
    Staining of human peripheral blood lymphocytes with Rat anti Human CD18:Biotin
  • FC - Anti Human CD18 Antibody, clone YFC118.3  ABD12671
    Staining of human peripheral blood lymphocytes with Rat anti Human CD18
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P05107
Reactivity Human
Host Rat
Clonality Monoclonal
Isotype IgG2b
Clone Names YFC118.3
Calculated MW 84782 Da
Additional Information
Other Species Dog,Gp
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Human neutrophils.
Shelf Life 18 months from date of despatch.
Gene ID 3689
Other Names Integrin beta-2, Cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 subunit beta, Complement receptor C3 subunit beta, CD18, ITGB2, CD18, MFI7
Target/Specificity Rat anti Human CD18 antibody, clone YFC118.3 was clustered at the Fourth International Workshop on Leucocyte Differentiation Antigen (code number N221) as recognising the CD18 antigen.The CD18 antigen is an integral membrane glycoprotein of ~95 kDa, known as the beta chain, of the LFA-1 complex. The CD18 antigen is non-covalently linked to CD11a, b, c molecules.The main cellular reactivity of Rat anti Human CD18 antibody, clone YFC118.3 is a strong reactivity with Leucocytes (platelets negative).The CD18 molecule is the receptor for ICAM-1 and is thus important for cell adhesion.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human CD18 Antibody, clone YFC118.3 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12671
(40 western blots)
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