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>   home   >   Products   >   Primary Antibodies   >   Anti Mouse Macrophages/Monocytes Antibody, clone MOMA-2    

Anti Mouse Macrophages/Monocytes Antibody, clone MOMA-2

Rat Anti-Mouse Monoclonal Antibody

     
  • FC - Anti Mouse Macrophages/Monocytes Antibody, clone MOMA-2  ABD12678
    Staining of mouse peritoneal macrophages with FITC conjugated Rat anti Mouse Macrophages/Monocytes following permeabilisation with Leucoperm
  • IHC-P - Anti Mouse Macrophages/Monocytes Antibody, clone MOMA-2  ABD12678
    Published customer image:Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 used for the detection of macrophages in aortic atherosclerotic lesions by immunohistochemistry on formalin fixed cryosections.Image caption:PBA treatment influenced the stability of plaque. Representative photomicrographs and the quantitative analysis of smooth muscle cell , CD3, macrophage (Mφ), and TUNEL in lesion of aortic root of ApoE-/- mice. The aortic root sections were stained with rabbit polyclonal to α- smooth muscle actin, rat anti-mouse macrophage Moma-2 and rat anti mouse CD3. The content of macrophage, smooth muscle cell, CD3 T cell in lesion was analyzed respectively by immunohistochemistry. For detection of cell apoptosis, sections were incubated with anti-TUNEL antibody and the content of apoptotic cells was analyzed by immunofluorescence. (n = 10 per group), *p<0.05, **p<0.01, ***p<0.001.From: Wang B, Dai S, Dong Z, Sun Y, Song X, Guo C, et al. The Modulation of Endoplasmic Reticulum Stress by Chemical Chaperone Upregulates Immune Negative Cytokine IL-35 in Apolipoprotein E-Deficient Mice.PLoS ONE 9(1): e87787.
  • IHC - Anti Mouse Macrophages/Monocytes Antibody, clone MOMA-2  ABD12678
    Published customer image:Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 used for the detection of mouse macrophages in atherosclerotic lesions by immunohistochemistry on cryosections.Image caption:Atherosclerotic lesions in aortic sinus. (A,B) Representative images of oil red O-stained atherosclerotic lesions in aortic sinus. (C,D) Immunohistochemical staining of macrophages in the cross-sections adjacent to the oil red O-stained images. (E) Quantitative analysis of the lesion area. Each dot indicates mean size of the oil red O-stained lesion area in each mouse. Bars indicate mean ± SEM of 8 mice for each group.From: Shuto Y, Asai A, Nagao M, Sugihara H, Oikawa S Repetitive Glucose Spikes Accelerate Atherosclerotic Lesion Formation in C57BL/6 Mice.PLoS ONE 10(8): e0136840.
  • IHC - Anti Mouse Macrophages/Monocytes Antibody, clone MOMA-2  ABD12678
    Published customer image:Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 used for the detection of macrophages in atherosclerotic aotic lesions from control and ApoE knockout mice.Image caption:MOMA-2 staining of ApoE-/- and ApoE-/-, P-selectin-/- mice fed a chow diet. Macrophages, stained by MOMA-2 monoclonal antibody, are shown binding the endothelial layer and penetrating the intimal lesions in SKO (a, c) and DKO (b, d) animals following, respectively, 15- (a, b) and 20- (c, d) week chow diet. Black arrows show immunostained macrophages. Original magnification: x250.From: Marie-Claude Bourdillon, Jacques Randon, Lydie Barek, et al., “Reduced Atherosclerotic Lesion Size in -Selectin Deficient Apolipoprotein -Knockout Mice Fed a Chow but Not a Fat Diet ,”Journal of Biomedicine and Biotechnology, vol. 2006, Article ID 49193, 8 pages, 2006.
  • IF - Anti Mouse Macrophages/Monocytes Antibody, clone MOMA-2  ABD12678
    Published customer image:Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 used for the detection of bone marrow derived macrophages in mouse by immunofluorescence.Image caption:ctivated Bone Marrow–Derived Macrophages Phagocytose Live Host Cells Confocal fluorescence microscopy. BMDMs are blue (F4–80 and MOMA-2), human T cells are green , and DNA is gray . (A–C) 30 min after the addition of Jurkats to BMDMs. (A) Unactivated BMDMs show little association with Jurkat cells, many of which were washed away. Scale bar is 40 μm (B and C). Activated macrophages phagocytose Jurkat cells; arrow denotes engulfed cell, arrowheads show partially engulfed cells. Scale bars are 40 μm (B) and 8 μm (C). (D–F) 42 h after mock-infection (D), or S. Typhimurium-infection (O-antigen, red, arrows, E and F). Scale bars are 20 µm (D), 40 µm (E), and 16 µm (F)..From: Nix RN, Altschuler SE, Henson PM, Detweiler CS Hemophagocytic Macrophages Harbor Salmonella enterica during Persistent Infection.PLoS Pathog 3: e193.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2b
Clone Names MOMA-2
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Mouse lymph node stroma.
Shelf Life 18 months from date of despatch.
Target/Specificity Rat anti Mouse Macrophages/Monocytes antibody, clone MOMA-2 recognizes an intracellular antigen of mouse macrophages and monocytes. It reacts strongly with macrophages in lymphoid organs such as tingible body macrophages and macrophages in T cell dependant areas and is extremely useful in immunohistochemistry. Reacts on all mouse strains tested.
Preservative & Stabilisers 0.09% Sodium Azide, 1% Bovine Serum Albumin
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Mouse Macrophages/Monocytes Antibody, clone MOMA-2 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12678
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