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>   home   >   Products   >   Primary Antibodies   >   Anti Rat MHC Class I RT1A Antibody, clone OX-18    

Anti Rat MHC Class I RT1A Antibody, clone OX-18

Mouse Anti-Rat Monoclonal Antibody

  • FC - Anti Rat MHC Class I RT1A Antibody, clone OX-18  ABD12679
    Staining of rat spleen cells with Mouse anti Rat MHC Class I Monomorphic: FITC
  • IHC-P - Anti Rat MHC Class I RT1A Antibody, clone OX-18  ABD12679
    Published customer image:Mouse anti Rat MHC Class I RT1A antibody, clone OX-18 used for the detection of RT1A expressing cells in mouse brain by immunofluorescence on formalin fixed tissue cryosections.Image caption:MHCI molecules are expressed in the nucleus accumbens core in WT mice. Staining for MHCI with OX-18 antibody , Neurogranin and the merged image are shown. Lower images represent negative controls without OX-18. Scale bar represents 50 µm.From: Edamura M, Murakami G, Meng H, Itakura M, Shigemoto R, et al. Functional Deficiency of MHC Class I Enhances LTP and Abolishes LTD in the Nucleus Accumbens of Mice.PLoS ONE 9(9): e107099.
  • FC - Anti Rat MHC Class I RT1A Antibody, clone OX-18  ABD12679
    Published customer image:FITC conjugated Mouse anti Rat MHC class I RT1A antibody used to evaluate MHC class I expression on rat mesenchymal stem cells by flow cytometry.Image caption: Immunophenotyping and pro-inflammatory cytokine expression of untransduced and Ad-transduced MSCs. (A) Untransduced and Ad-transduced MSCs were stained for expression of the immunologically relevant markers MHC class I/II, CD80 and CD86 with specific antibodies and analysed by flow cytometry. (B) MSCs were transduced with Ad.GFP and after 24 h cells were collected and subjected to mRNA isolation and cDNA synthesis. RT-PCR analysis showing mRNA expression levels of IL-1ß, IFN-? and IL-6 from untransduced and Ad-transduced MSCs. Data shown are means ±SD of three independent experiments. 2E-?CT = 2-?CT? number of copies of gene of interest relative to the number of copes of the internal control gene, ß-actin.From: Treacy O, Ryan AE, Heinzl T, O'Flynn L, Cregg M, et al. Adenoviral Transduction of Mesenchymal Stem Cells: In Vitro Responses and In Vivo Immune Responses after Cell Transplantation.PLoS ONE 7(8): e42662.
  • IHC - Anti Rat MHC Class I RT1A Antibody, clone OX-18  ABD12679
    Published customer image:Mouse anti Rat MHC class I RT1A antibody, clone OX-18 used for the detection of MHC classI expressing cells in rat brain by immunohistochemistry of formalin fixed tissue cryosections.Image caption:Expression of major histocompatibility complex class I and class II antigens in the right injected striatum. Photomicrographs were prepared from coronal sections through the striatum and processed for MHC class I (A–D) and MHC class II (F–I) immunocytochemistry in representative rats of the M PBS-Str hEPO (A and F), M hEPO-Str hEPO (B and G), Str PBS-Str hEPO (C and H) and Str hEPO-Str hEPO (D and I) groups 3 weeks after the second injections. The scale bar = 100 µm in I also for A–D and F–H. High magnification photomicrographs in the left bottom were prepared from the box areas in (A) and (H). (E) and (J) Arbitrary rating scores of MHC class I (E) and MHC class II (J) immunostaining in the right injected striatum of rats from the M PBS-Str hEPO, M hEPO-Str hEPO, Str PBS-Str hEPO and Str hEPO-Str hEPO groups. The bars represent the median values of rating scores, and the circles depict the individual values. Filled circles indicate the rats from which the photomicrographs were taken. A Kruskal-Wallis test followed by Mann-Whitney U tests was used to compare the rating scores between groups. * p
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names OX-18
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Rat spleen cell glycoproteins
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti Rat MHC Class I RT1A antibody, clone OX-18 recognizes a monomorphic determinant of rat MHC Class I (RT1A), expressed by all rat strains. However, quantitative measurements suggest that not all of the class I molecules are recognized.Mouse anti Rat MHC Class I RT1A antibody, clone OX-18 has been used in immunoaffinity purification of rat MHC class I molecules (Fukumotoet al.1982).Mouse anti Rat MHC Class I RT1A antibody, clone OX-18 is routinely tested in flow cytometry on rat splenocytes.
Preservative & Stabilisers 0.09% Sodium Azide, 1% Bovine Serum Albumin
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Rat MHC Class I RT1A Antibody, clone OX-18 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12679
(40 western blots)
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