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Anti Human CD34 Antibody, clone QBEND/10

Mouse Anti-Human Monoclonal Antibody

  • IF - Anti Human CD34 Antibody, clone QBEND/10  ABD12684
    Published customer image:Mouse anti Human CD34 antibody, clone QBEND/10 used for the detection of CD34 positive cells in vitro by immunofluorescence.Image caption:Characterization of human placental multipotent mesenchymal stromal cells after inducing differentiation into endothelial cells. Immunofluorescence staining of CD31 in undifferentiated hPMSCs is shown in (A) as a negative control. Immunofluorescence of endothelial markers in differentiated hPMSCs under induced culture conditions: (B) CD31, (C) CD34, (D) VE-cadherin, (E) VEGFR-1, (F) VEGFR-2, (G) von Willebrand factor, (H) von Willebrand factor under high magnification (Scale bar: 1 µm); Weibel-Palade bodies were visible within the differentiated hPMSC cytoplasm . CD105 was positive in (I) undifferentiated hPMSCs and (J) hPMSCs differentiated into endothelial cells. Cell nuclei were counterstained with DAPI. Scale bar: 10 µm. (K) Flow cytometry analysis of endothelial cell markers on the hPMSC surface before and after differentiation induced by VEGF-A. (L) mRNA for endothelial cell markers was amplified from 2 different strains of undifferentiated hPMSCs (lane 1 and 2) and hPMSCs differentiated into endothelial cells (lane 3 and 4). mRNA from human umbilical vein endothelial cells was used as a positive control (lane 5). The data shown are representative of 3 different experiments. From: Lee M-Y, Huang J-P, Chen Y-Y, Aplin JD, Wu Y-H, et al. Angiogenesis in Differentiated Placental Multipotent Mesenchymal Stromal Cells Is Dependent on Integrin a5ß1. PLoS ONE 4: e6913.
  • IHC - Anti Human CD34 Antibody, clone QBEND/10  ABD12684
    Published customer image:Mouse anti Human CD34 antibody used to identify CD34 positive cells in cancer tissue microarrays bu immunohistochemistry.Image caption: Examples of immunostained RCC group-specific markers CD34, DEK and MSH6. ccRCC with CD34-stained vascular microvessels (A, B); ccRCC with strong nuclear DEK (C) and MSH6 (D) positivity.From: Beleut M, Zimmermann P, Baudis M, Bruni N, Bühlmann P, Laule O, Luu VD,Gruissem W, Schraml P, Moch H. Integrative genome-wide expression profilingidentifies three distinct molecular subgroups of renal cell carcinoma withdifferent patient outcome. BMC Cancer. 2012 Jul 23;12:310.
  • FC - Anti Human CD34 Antibody, clone QBEND/10  ABD12684
    Paraffin processed placenta stained with Mouse anti Human CD34 Class II
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P28906
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names QBEND/10
Calculated MW 40716 Da
Additional Information
Other Species B,Cynomolgus monkey,Rh
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Human endothelial cell membrane vesicles.
Shelf Life 18 months from date of despatch.
Gene ID 947
Other Names Hematopoietic progenitor cell antigen CD34, CD34, CD34
Target/Specificity Mouse anti Human CD34 antibody, clone QBEND/10 recognizes the human CD34 antigen, also known as Hematopoietic progenitor cell antigen CD34. Human CD34 is 385 amino acid polypeptide containing a 31 residue signal peptide, cleaved to yield the ~110kDa mature form of CD34, a sialomucin single pass transmembrane glycoprotein. CD34 is expressed by stem cells (Kaufmanet al.2001) and small vessel endothlium (Ramaniet al.1990)Human CD34 exists as two isoforms, the full length form described here and a truncated isoform lacking the carboxy-terminal of the intracellular domain and containing some alternative sequence in the remaining intracellular region. Antibody binding epitopes on human CD34 have been classified according to their resistance to enzymatic degradation and grouped together using this and competitive binding assays (Lanzaet al.1999). Clone QBEND/10 has been classified as binding to the class II epitope, resistant to neuraminidase treatment but sensitive to both glycoprotease and chymopapain digestion. Clone QBEND/10 binds to a different eoptope to clone 581 which binds to the class III epitope resistant to all three enzymzatic treatments (Nishio et al. 1996 In Leukocyte tTyping VI). Clone QBEND 10 is expected to bind to both isoforms of human CD34 as it's binding epitope has been mapped to the extracellular domain between amino acids 43 and 49 by peptide microarray analysis (Jones et al. 1996, in Leukocyte Typing VI).Mouse anti Human CD34 antibofy, clone QBEND/10 has been succesfully exploited foir the detection of CD34 in brain capillaries of Alzheimer's patients (Kalariaet al.1992) and in acute lymphoblastic leukemia cells (Sutherlandet al.1992) by western blotting.
Preservative & Stabilisers 0.09% Sodium Azide, 1% Bovine Serum
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human CD34 Antibody, clone QBEND/10 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12684
(40 western blots)
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