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Anti Human CD43 Antibody, clone DFT-1

Mouse Anti-Human Monoclonal Antibody

     
  • FC - Anti Human CD43 Antibody, clone DFT-1  ABD12688
    Imunofluorescence staining of human tonsil cryosection with Mouse anti Human CD43, clone DFT-1 , red in A and Mouse anti Human CD21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power
  • IHC - Anti Human CD43 Antibody, clone DFT-1  ABD12688
    Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD43 antibody, clone DFT-1 followed by the HISTAR detection system . Medium power
  • FC - Anti Human CD43 Antibody, clone DFT-1  ABD12688
    Imunofluorescence staining of human tonsil cryosection with Mouse anti Human CD43, clone DFT-1 , red in A and Mouse anti Human CD21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Medium power
  • FC - Anti Human CD43 Antibody, clone DFT-1  ABD12688
    Staining of human peripheral blood lymphocytes with Mouse anti Human CD43:Azide Free
  • IHC - Anti Human CD43 Antibody, clone DFT-1  ABD12688
    Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD43 antibody, clone DFT-1 followed by the HISTAR detection system . Low power
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IHC-F, FC
Primary Accession P16150
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names DFT-1
Calculated MW 40322 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen KG-1 cells.
Shelf Life 18 months from the date of despatch
Gene ID 6693
Other Names Leukosialin, Galactoglycoprotein, GALGP, Leukocyte sialoglycoprotein, Sialophorin, CD43, SPN, CD43
Target/Specificity Mouse anti Human CD43 antibody, clone DFT-1 recognizes human CD43 also known as leukosialin or sialophorin. CD43 is a 381 amino acid single pass type I transmembrane glycoprotein present on the cell surface of multiple cell types including thymocytes, T-lymphocytes, neutrophils, plasma cells and platelets. It is lacking on the surface of most B-lymphocytes. CD34 is a major membrane sialoglycoprotein expressed on T lymphocytes as two predominant glycoforms, a 115kDa form present on all T-cells and a 130kDa form indicitive of T-cell activation (Barrenet al.1997) and acts as a T-cell counter-receptor for CD169/Siglec-1(van den Berg et al.2001). Clone DFT-1 binds to both glycoforms of human CD43 (Kyoizumiet al.2004)CD34 expression is deficient and/or defective in patients suffering from the X-linked chromocomal immunodeficiency condition Wiskott-Aldrich syndrome and acts as a ligand for CD54(Rosensteinet al.1991). Mouse anti human CD43, clone DFT-1 has been used successfully for the evaluation of CD43 expression in patients with Myelodysplastic syndromes (Kyriakouet al.2006)
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human CD43 Antibody, clone DFT-1 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12688
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(40 western blots)
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