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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti Rat CD4 (Domain 1) Antibody, clone W3/25    

Anti Rat CD4 (Domain 1) Antibody, clone W3/25

Mouse Anti-Rat Monoclonal Antibody

     
  • IF - Anti Rat CD4 (Domain 1) Antibody, clone W3/25  ABD12690
    Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. High power
  • IF - Anti Rat CD4 (Domain 1) Antibody, clone W3/25  ABD12690
    Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. Low power
  • IF - Anti Rat CD4 (Domain 1) Antibody, clone W3/25  ABD12690
    Published customer image:Dynamics of T-regulatory cells. D–F) Photographs of double immunolabelled sections showing a representative CD4+Foxp3+ cell found around blood vessels . Bar scale = 30 µm.From: Almolda B, Costa M, Montoya M, González B, Castellano B Increase in Th17 and T-reg Lymphocytes and Decrease of IL22 Correlate with the Recovery Phase of Acute EAE IN Rat. PLoS ONE 6: e27473.
  • IF - Anti Rat CD4 (Domain 1) Antibody, clone W3/25  ABD12690
    Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. Medium power
  • FC - Anti Rat CD4 (Domain 1) Antibody, clone W3/25  ABD12690
    Staining of stimulated rat spleen cells with Mouse anti Rat CD4
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Primary Accession P05540
Reactivity Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names W3/25
Calculated MW 51438 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Rat Thymocyte Membrane Glycoproteins
Shelf Life 18 months from date of despatch.
Gene ID 24932
Other Names T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, W3/25 antigen, CD4, Cd4
Target/Specificity Mouse anti Rat CD4 antibody, clone W3/25 recognizes the rat CD4 cell surface glycoprotein, a 55kD molecule expressed by helper T cells and weakly by monocytes. This antibody inhibits proliferation and IL-2 production in the MLR reaction.Mouse anti Rat CD4 antibody, clone W3/25 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) (Whitelandet al.1995).Mouse anti Rat CD4 antibody, clone W3/25 is routinely tested in flow cytometry on rat splenocytes
Preservative & Stabilisers 0.09% Sodium Azide (NaN3),Sucrose
Storage Store at +4℃. DO NOT FREEZE.
PrecautionsAnti Rat CD4 (Domain 1) Antibody, clone W3/25 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12690
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