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Anti Human Apolipoprotein E Antibody, clone WUE-4

Mouse Anti-Human Monoclonal Antibody

  • E - Anti Human Apolipoprotein E Antibody, clone WUE-4  ABD12692
    Sandwich ELISA analysis of human apolipoprotein E using Mouse anti Human apolipoprotein E as a capture reagnt and biotinylated Goat anti Human Apolipoprotein E as a detection reagent with purified apolipoprotein E as antigen. Detection is with Streptavidin HRP. A serum sample 1/150 and a plasma sample 1/150 are shown. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader
  • E - Anti Human Apolipoprotein E Antibody, clone WUE-4  ABD12692
    Human apolipoprotein E detected with Mouse anti Human apolipoprotein E:HRP
  • E - Anti Human Apolipoprotein E Antibody, clone WUE-4  ABD12692
    Human apolipoprotein E detected with Mouse anti Human apolipoprotein E followed by Rabbit F(ab')2 anti Mouse IgG:HRP
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P02649
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names WUE-4
Calculated MW 36154 Da
Additional Information
Other Species Sea Lion,M
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Purified ApoHDL fraction.
Shelf Life 18 months from date of despatch.
Gene ID 348
Other Names Apolipoprotein E, Apo-E, APOE
Target/Specificity Mouse anti Human Apolipoprotein E antibody, clone WUE-4 recognizes an epitope within amino acids 140-160 of human apolipoprotein E (Apo-E), a major component of very low-density lipoproteins (VLDLs). Apo-E is the principle apolipoprotein in the central nervous system, and is secreted by most organs into the plasma, playing a vital role in the binding, internalization and catabolism of triglyceride-rich lipoprotein constituents.Apo-E acts as a ligand for both the specific apo-E receptor (chylomicron remnant) of hepatic tissues, and the apoB,E (LDL) receptor. Three isoforms of Apo-E have been identified, ApoE2, E3 and E4, and have been linked with various disorders. ApoE2 has been shown to bind LPL receptors with low affinity, resulting in increased plasma cholesterol and triglyceride levels, and thereby an increased risk in cardiovascular disorders. ApoE4 is a known high risk factor for Alzheimers disease, and in particular late onset Alzheimer disease 2 (AD2), whilst ApoE3 is the most common isoform, and considered the normal/natural Apo-E genotype.Mouse anti Human Apolipoprotein E antibody, clone WUE-4 has been shown to inhibit Apo-E mediated binding of lipoproteins to the apoB,E cell receptor (Krul et al. 1998).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human Apolipoprotein E Antibody, clone WUE-4 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12692
(40 western blots)
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