Anti Human Glutamate Decarboxylase 2 (N-Terminal) Antibody, clone N-GAD65
Mouse Anti-Human Monoclonal Antibody
|Application ||WB, IHC-P, IP|
|Calculated MW||65411 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||Keyhole Limpet Haemocyanin (KLH) conjugated synthetic peptide sequence PGSGFWSFGSEDGSGDSEN corresponding to amino acids 4-22 within the N-terminal region of human GAD65.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Glutamate decarboxylase 2, 188.8.131.52, 65 kDa glutamic acid decarboxylase, GAD-65, Glutamate decarboxylase 65 kDa isoform, GAD2, GAD65|
|Target/Specificity||Mouse anti Human Glutamate Decarboxylase 2 (N-Terminal) antibody, clone N-GAD65 recognizes human glutamate decarboxylase 2, also known as 65 kDa glutamic acid decarboxylase, Glutamate decarboxylase 65 kDa isoform or GAD-65. Glutamate decarboxylase 2 is a 585 amino acid ~65 kDa lipid anchored membrane associated protein responsible for catalysis of γ-aminobutyric acid (GABA) production, the major inhibitory neurotransmitter in the central nervous system (Fenaltiet al.2007) and is expressed in the brain and pancreatic beta cells. Glutamate decarboxylase 2 is the 65 kDa isoform of glutamate decarboxylase, encoded by the GAD2 gene and predominantly expressed by nerve termini, as opposed to the 67 kDa isoform (GAD67), predominantly found in the cell body, and encoded by the GAD1 gene. Autoantibodies to Glutamate decarboxylase 2 are detected in autoimmune diseases, including Graves disease (Kallmannet al.1997), Stiff Man Syndrome (Rajuet al.2005) and are most prevalent in patients with Type I diabetes mellitus or those at high risk of developing Type I diabetes (Petersenet al.1994). The N-Terminal region of GAD65 is essential for targeting the enzyme to GABA-containing secretory vesicles (Shiet al.1994).Mouse anti Human Glutamate Decarboxylase 2 (N-Terminal) antibody, clone N-GAD65 recognizes GAD65 but does not recognize GAD67 (Hampe et al.2001).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20℃ if preferred.|
|Precautions||Anti Human Glutamate Decarboxylase 2 (N-Terminal) Antibody, clone N-GAD65 is for research use only and not for use in diagnostic or therapeutic procedures.|
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