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>   home   >   Products   >   Primary Antibodies   >   Anti Bovine MHC Class II DR Antibody, clone CC108    

Anti Bovine MHC Class II DR Antibody, clone CC108

Mouse Anti-Bovine Monoclonal Antibody

     
  • FC - Anti Bovine MHC Class II DR Antibody, clone CC108  ABD12702
    Published customer image:Mouse anti Bovine MHC class II DR antibody, clone CC108 used for the evaluation of MHC class II expression on peripheral blood cells by flow cytometry.Image caption:CD8a- SIRPa- ALDCs express cytokines that induce ?d T with a regulatory phenotype. Phenotypic analysis of bovine ALDC subsets includes FSChigh MHC class II (A), SIRPa and CD11c (B), and SIRPa and CD8a (C). Cells were fixed, permeabilized, and stained for intracellular TGF-ß and IL-10. DCs were gated on SIRPa+ CD8a- (D), SIRPa+ CD8a+ (E), and SIRPa- CD8a- double negative (F). Dot plots are representative of cells from five different animals. (G–I) SIRPa- CD8a- ALDCs are capable of inducing ?d T cells with suppressive phenotype. (G) Subpopulations of ALDCs were FACS sorted and cocultured with autologous MACS-sorted ?d TCR+ T cells for 5 d. Proliferation was measured by CFSE dilution and intracellular staining of IL-10 by flow cytometry. (H) FMDV-specific proliferation of CD4+ T cells in the absence or presence of in vitro–expanded autologous ?d T cells by SIRPa- CD8a- ALDCs and in Transwell plates with or without blocking anti–IL-10. (I) FMDV-specific IFN-? responses in CD4+ T cells in the presence or absence of in vitro–expanded autologous ?d T cells SIRPa- CD8a- ALDCs and in Transwell plates with or without blocking anti–IL-10. Bars indicate means of cells from four different animals analyzed in triplicate, and error bars indicate SEMs.From: Guzman E, Hope J, Taylor G, Smith AL, Cubillos-Zapata C, Charleston B.Bovine γδ T cells are a major regulatory T cell subset.J Immunol. 2014 Jul 1;193(1):208-22.
  • FC - Anti Bovine MHC Class II DR Antibody, clone CC108  ABD12702
    Published customer image:Mouse anti Bovine MHC Class II DR antibody, clone CC108 used for the evaluation of MHC class II expression on bovine myeloid cell sub-populations. Image caption:Phenotypic profiles of bovine myeloid cell sub-populations. Live gated PBMC were assessed for expression of CD16 and CD14 and a panel of molecules associated with antigen presentation, co-stimulation or specific cell lineages (see Table 1) by 3 colour flow cytometry. PBMC were stained with primary mAb to the specific molecules indicated and then with an isotype-specific PE conjugated secondary, followed by CD16 and CD14 conjugated to FITC or Alexa Fluor 647 respectively. Live, single PBMC were gated based on the expression of CD16 and CD14 as detailed in Figures 1 and ?and 2C.2C. Histograms show the levels of expression of selected markers in the cell populations studied, CD14-CD16++ , CD14+CD16+ and CD14+CD16low/- compared to PBMC . Data shown are for one representative animal of four animals.From: Corripio-Miyar Y, Hope J, McInnes CJ, Wattegedera SR, Jensen K, Pang Y,Entrican G, Glass EJ.Phenotypic and functional analysis of monocyte populations in cattle peripheral blood identifies a subset with high endocytic and allogeneicT-cell stimulatory capacity.Vet Res. 2015 Sep 25;46:112.
  • FC - Anti Bovine MHC Class II DR Antibody, clone CC108  ABD12702
    Bovine peripheral lymphocytes stained with Mouse anti Bovine MHC Class II DR followed by Goat anti Mouse IgG (H/L):FITC
  • FC - Anti Bovine MHC Class II DR Antibody, clone CC108  ABD12702
    Published customer image:Mouse anti Bovine MHC class II DR antibody, clone CC108 used for the evaluation of MHC class II expression on peripheral blood cells by flow cytometry.Image caption:Monocyte/macrophages and MoDCs express IL-10 and TGF-β, and induce proliferation of IL-10+ γδ T cells. Peripheral blood monocyte/macrophages expressing CD14 (A) and expression of MHC class II/CD1b (B) in CD14+ cells. After a 3-d culture in the presence of GM-CSF and IL-4, the cells increased expression of MHC class II and CD1b (C). IL-10 and TGF-β expression in ex vivo CD14+ cells (D) and cultured MoDCs (E). Both monocyte/macrophages and MoDCs induced the expansion of autologous IL-10–expressing γδ T cells (F). Bar graph shows means (n = 10) and error bars indicate SEMs. Representative plots of cells obtained from 10 different animals analyzed in duplicate. Quadrants were placed based on isotype and fluorochrome controls.From: Guzman E, Hope J, Taylor G, Smith AL, Cubillos-Zapata C, Charleston B.Bovine γδ T cells are a major regulatory T cell subset.J Immunol. 2014 Jul 1;193(1):208-22.
  • FC - Anti Bovine MHC Class II DR Antibody, clone CC108  ABD12702
    Bovine peripheral lymphocytes stained with Mouse anti Bovine MHC Class II DR:RPE
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Reactivity Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names CC108
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Shelf Life 18 months from the date of despatch
Target/Specificity Mouse anti Bovine MHC class II DR antibody, clone CC108 recognizes Bovine MHC Class II DR. MHC Class II molecules are constitutively expressed on antigen presenting cells such as dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of other cell types by interferon gamma.The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In cattle, this complex is referred to as the bovine leukocyte antigen (BoLA) region. There are 2 major types of MHC class IIa molecules encoded by the BoLA which are DR and DQ each composed of an alpha and beta chain.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Bovine MHC Class II DR Antibody, clone CC108 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12702
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