|Application ||WB, IHC-P, E|
|Calculated MW||92670 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||Recombinant human HIF1 alpha|
|Shelf Life||18 months from the date of despatch.|
|Other Names||Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, HIF1A, BHLHE78, MOP1, PASD8|
|Target/Specificity||Mouse anti Human HIF1 alpha antibody, clone ESEE122 recognizes human HIF1α, also known as Hypoxia-inducible factor 1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Member of PAS protein 1 or PAS domain-containing protein 8. HIF1α is a widely expressed bHLHandPAS domain containing transcription factor, a critical regulatory protein in the host response to hypoxia.HIF1α interacts with the HIF1β/ARNT subunit to form the highly-conserved heterodimeric HIF-1 transcriptional complex. During hypoxic conditions, HIF1α translocated from a normoxic cytoplasmic location to the nucleus and activates transcription of several genes including erythropoietin, VEGF and glucose transporters, encoding proteins involved in oxygen delivery and metabolic adaptation to hypoxia. Under normal oxygen conditions, HIF1α is targeted by HIF prolyl-hydroxylase, followed by rapid proteolytic degradation. Overexpression of HIF1α occurs in many common human cancers, including pancreatic, bladder and renal carcinomas (Semenza 2003).Mouse anti Human HIF1 alpha antibody, clone ESEE122 has been shown to be suitable for the staining of snap-frozen cell pellets (Talks.et al.2000).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20℃ if preferred.|
|Precautions||Anti Human HIF1 Alpha Antibody, clone ESEE122 is for research use only and not for use in diagnostic or therapeutic procedures.|
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