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Anti Human Calcitonin Receptor Antibody, clone 30/7-9B4

Mouse Anti-Human Monoclonal Antibody

  • IHC-P - Anti Human Calcitonin Receptor Antibody, clone 30/7-9B4  ABD12763
    Published customer image:Mouse anti Human Calcitonin Receptor antibody, clone 30/7-9B4 used for the identification of calcitonin receptor expressing cells in bovine cartilage explants by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Immunolocalization of the calcitonin receptor in cartilage explants induced with or without T3 for 4 days. First panel shows the superficial layer of the cartilage, second panel the mid layer of the cartilage and the third panel the deep layer of the cartilage. Treatment scheme is indicated at the top of the pictures. The pictures are representative of 3 replicates from the same experiment. Positive staining is visualized by brown color.From: Chen-An P, Andreassen KV, Henriksen K, Li Y, Karsdal MA, Bay-Jensen A-C The Inhibitory Effect of Salmon Calcitonin on Tri-Iodothyronine Induction of Early Hypertrophy in Articular Cartilage.PLoS ONE 7(6): e40081.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P30988
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names 30/7-9B4
Calculated MW 59352 Da
Additional Information
Other Species Rb,B
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Synthetic peptide derived from sequence situated on the extra-cellular domain of human calcitonin receptor.
Shelf Life 18 months from date of despatch.
Gene ID 799
Other Names Calcitonin receptor, CT-R, CALCR
Target/Specificity Mouse anti Human Calcitonin Receptor antibody, clone 30/7-9B4 recognizes the human calcitonin receptor, a 508 amino acid multi pass transmembrane membrane glycoprotein with seven transmembrane domains, coupled to G protein messenger systems. The calcitonin receptor is expressed in kidney, central and peripheral nervous systems and on osteoclasts. It is also expressed in a number of tissues during the development of the embryo and may be important in foetal morphogenesis.Clone MAb 9B4 recognises an epitope within the extra-cellular domain, the epitope is common to both C1a and C1b human isoforms.Calcitonin receptor expression is elevated in the inflammatory cells associated with diseased arteries and atherosclerotic plaques (Wookeyet al.2009) expression has also been noted within malignant cells of glioblastoma multiforme (Wookeyet al.2012).Several different isoforms of the calcitonin receptor, a result of alternative splicing and promotor usage have been identified. Mouse anti Human Calcitonin Receptor antibody, clone 30/7-9B4 was raised against the canonical isoform 1 but has been shown to bind to a truncated isoform lacking the N-terminal region of the receptor (Qiet al.2012). The calcitonin receptor can associate with RAMP1, 2 and 3 to form discrete receptors for amylinMouse anti human calcitonin receptor antibody, clone 30/7-9B4 has been successfully used for the demonstrating calcitonin receptor on human cryostat tissue section by immunohistochemistry and western blotting (Wookeyet al.2012).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human Calcitonin Receptor Antibody, clone 30/7-9B4 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12763
(40 western blots)
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