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>   home   >   Products   >   Primary Antibodies   >   Anti Chicken Monocytes/Macrophages Antibody, clone KUL01    

Anti Chicken Monocytes/Macrophages Antibody, clone KUL01

Mouse Anti-Chicken Monoclonal Antibody

  • FC - Anti Chicken Monocytes/Macrophages Antibody, clone KUL01  ABD12775
    Published investigator image:Mouse anti Chicken monocytes/macrophages antibody, clone KUL01 used for flow cytometry studies.Image caption:Immunohistochemical evidence of infiltration by T-cells (CD3+), B-cells (chB6+) and macrophages (Kul01+). Immunophenotyping identified the majority of infiltrating lymphocytes as being CD3-positive T-cells (A). They show a characteristic multifocal distribution pattern. With similar spatial characteristics, chB6-positive B-cells comprise the second largest fraction of infiltrating cells (B). Compared to the lymphocytes, the density of Kul01-positive macrophages appears much lower (C). The arrows point to immunopositive cells. All sections were prepared from the same region of the sciatic nerve. The orientation of the nerve fibres is indicated. Scale bar = 35 µm.From: Bader SR, Kothlow S, Trapp S, Schwarz SC, Philipp HC, Weigend S, Sharifi AR, Preisinger R, Schmahl W, Kaspers B, Matiasek K. Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans. J Neuroinflammation. 2010 Jan 28;7:7.
  • FC - Anti Chicken Monocytes/Macrophages Antibody, clone KUL01  ABD12775
    Published customer image:Mouse anti Chicken monocytes/macrophages antibody, clone KUL01 used for flow cytometry studies.Image caption:Expression of fluorescent protein reporter in MPs requires integration of a full length CSF1R-reporter construct containing chicken FIRE. (A) Southern blot analysis of G1 progeny from G0 founder MAA2-16. DNA isolated from the blood of four G1 birds (lanes 1-4) were digested with restriction enzymes XhoI and ClaI isolate the full-length insert and probed with sequence mApple coding sequence. Expected size of full length insert (5.5 kb) was observed in G1 progeny MAA2-16:13 and MAA2-16:22 (lane 1 and 3), whereas a partially-deleted insert was found in G1 progeny MAA2-16:20 and MAA2-16:33. (B) PCR analysis of G2 progeny (lanes 1-12) from G1 cockerel MAA1-8:23 for the presence of full length (2.5 kb) or partially-deleted (0.5 kb) inserts using primers P1 and P2. (C) Flow cytometric analysis of reporter transgene expression in KUL01+/CSF1R+ blood monocytes in a non-transgenic chicken, G1 cockerel MAA1-8:23 and representative G2 progeny from (B) that have either a full length or a partially-deleted insert. From: Balic A, Garcia-Morales C, Vervelde L, Gilhooley H, Sherman A, Garceau V, Gutowska MW, Burt DW, Kaiser P, Hume DA, Sang HM. Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage. Development. 2014 Aug;141:3255-65.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Chicken
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names KUL01
Additional Information
Purification Purified IgG prepared by ion exchange chromatography
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti Chicken Monocytes/Macrophages, clone KUL01 recognises chicken monocytes and macrophages. Clone KUL01 will also detect interdigitating cells and activated microglia but does not recognise Bu1+ve B-cells or CD3 +ve T-lymphocytes.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Chicken Monocytes/Macrophages Antibody, clone KUL01 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12775
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