Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Loading Control   >   Anti Human Actin Beta Antibody, clone 4C2    

Anti Human Actin Beta Antibody, clone 4C2

Mouse Anti-Human Monoclonal Antibody

     
  • WB - Anti Human Actin Beta Antibody, clone 4C2  ABD12780
    Published customer image:Mouse anti Human Actin beta antibody, clone 4C2 used for the evaluation of γ-actin expression in cell lines by immunofluorescence and western blotting.Image caption:β- and γ-actins in neoplastic cells phenotype A. WB analysis of A549 cells (left panel) and HCT116 cells (right panel) with exogenous expression of β- or γ-actins and corresponding shRNAs. Graphs represent relative actins expression (Mean ± SD). B. Semi-quantitative RT-PCR analysis of β- or γ-actins expression in A549 (left panel) and HCT116 (right panel) with corresponding shRNAs. Graphs represent folds of RNA expression in comparison to control cells (Mean ± SD). C. Immunofluorescent staining of A549 cells (left panel) and HCT116 cells (right panel) with exogenous expression of β- or γ-actins and corresponding shRNAs. Scale bars represent 10 μm.From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.Oncotarget. 2015 Jun 10;6:14556-71.
  • IF - Anti Human Actin Beta Antibody, clone 4C2  ABD12780
    Published customer image:Mouse anti Human actin antibody, clone 4C2 used for the demonstration of actin expression in epithelial cells by immunofluorescence.Image caption:Normal and transformed IAR epithelial cells. (A) Single IAR-2 epithelial cells are discoid, they form islands in sparse culture and a confluent monolayer in dense culture. Scale bar 10 µm. (B) E-cadherin and N-cadherin expression in normal and transformed IAR cells. (C) IAR-2 cells were stained for E-cadherin and actin . Top row (1.25 µm above the substrate) shows AJs organized as adhesion belts encircling each cell and co-localizing with circumferential actin bundles in the apical parts of cells. Bottom row (substrate level) shows parts of adhesion belts and irregular distributions of actin. Scale bar 10 μm. (D) Transformed IAR-6-1 cells were stained for E-cadherin and actin . In these cells, radial AJs were associated with thin actin bundles. Scale bar 10 μm.From: Rubtsova SN, Zhitnyak IY, Gloushankova NA A Novel Role of E-Cadherin-Based Adherens Junctions in Neoplastic Cell Dissemination.PLoS ONE 10(7): e0133578.
  • IF - Anti Human Actin Beta Antibody, clone 4C2  ABD12780
    Published customer image:Mouse anti Human Actin beta antibody, clone 4C2 used for the evaluation of γ-actin expression in A549 cells by immunofluorescence and western blotting.Image caption:γ-actin and ERK1/2 are mutually regulated A. WB analysis of A549 cells with exogenous expression of β- or γ-actins and corresponding shRNAs. Graphs represent relative pERK1/2 expression (Mean ± SD). B. LSM of A549 cells with down-regulated β- or γ-actins with β-actin , γ-actin or pERK1/2 immunofluorescent staining. Scale bars represent 10 μm. C. pERK1/2 immunoprecipitation analysis of A549 cells with down-regulated β- or γ-actins. D. pERK1/2/γ-actin PLA analysis of A549 cells with down-regulated β- or γ-actins. Scale bar represents 10 μm. Graph represents relative fluorescence intensity (Mean ± SD). E. LSM of A549 cells treated with EGF or UO126 with β-actin or γ-actin immunofluorescent staining. Scale bars represent 10 μm. F. WB analysis of A549 cells treated with EGF or UO126. Graphs represent relative pERK1/2 or β-/γ-actin levels (Mean ± SD).From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.Oncotarget. 2015 Jun 10;6:14556-71.
  • IF - Anti Human Actin Beta Antibody, clone 4C2  ABD12780
    Published customer image:Mouse anti Human Actin beta; antibody, clone 4C2 used for the evaluation of γ-actin expression in A549 cells by immunofluorescence and western blotting.Image caption:γ-actin co-localizes with PP1 and regulates it A. LSM of A549 cells with down-regulated β- or γ-actins with β-actin , γ-actin , PP1α or DAPI immunofluorescent staining. Scale bars represent 10 μm. B. WB analysis of A549 cells with down-regulated β- or γ-actins. Graphs represent relative PP1&alpha levels (Mean ± SD). C. PP1 immunoprecipitation analysis of A549 cells with down-regulated β- or γ-actins. D. PP1α/γ-actin (upper panel) and PP1α/β-actin PLA analysis of A549 cells with down-regulated β- or γ-actins. Scale bar represents 10 μm. E. Comparative quantification of PP1α-β-/γ-actin PLA dots. Graph represents relative fluorescence intensity (Mean ± SD).From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.Oncotarget. 2015 Jun 10;6:14556-71.
  • E - Anti Human Actin Beta Antibody, clone 4C2  ABD12780
    Sandwich ELISA analysis of human actin beta binding using Human anti Human actin beta antibody, clone 12141 as a capture reagent and biotinylated Mouse anti Human actin, clone 4C2 as a detection reagent with purified human actin beta as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader . Asthmatic sufferer serum samples (orange and blue) and SLE serum sample are shown. Beta actin was undetectable in normal serum samples
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-F, IF
Primary Accession P60709
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 4C2
Calculated MW 41737 Da
Additional Information
Other Species Rb,Pig,M,Rat
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Synthetic peptide corresponding to the acetylated N-terminal nonapeptide of beta-cytoplasmic actin, coupled to KLH.
Shelf Life 18 months from date of despatch.
Gene ID 60
Other Names Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB
Target/Specificity Mouse anti Human actin beta, clone 4C2, recognises cytoplasmic actin beta (known also as ACTB and Beta Actin), a 41.7 kDa cytoskeletal protein. Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified in mammals (Vandekerckhoveet al.1978).Clone 4C2 is highly specific for cytoplasmic actin β and does not react with other actin isoforms. Mouse anti Human actin beta, clone 4C2 has been used to look at the remodeling of the actin cytoskeleton following sendai virus infection and the specific role of the viral M protein in actin remodelling (Miazzaet al.2011)
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human Actin Beta Antibody, clone 4C2 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to
info@abgent.com, and receive a free "I Love Antibodies" mug.
FeedBack
Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abgent.com.

Cat# ABD12780
Size:
Quantity:
Availability: Inquire
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Crown Flash17-30% Off
Terms & Conditions