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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti Human Actin Gamma Antibody, clone 2A3    

Anti Human Actin Gamma Antibody, clone 2A3

Mouse Anti-Human Monoclonal Antibody

     
  • IHC - Anti Human Actin Gamma Antibody, clone 2A3  ABD12781
    Published customer image:Mouse anti Human Actin gamma antibody, clone 2A3 used for the evaluation of γ-actin expression in A549 cells by immunofluorescence and western blotting.Image caption:γ-actin co-localizes with p34-Arc and WAVE2 and regulates them A. p34-Arc or γ-actin immunofluorescence images of A549 cells with down-regulated β- or γ-actins. Scale bars represent 10 μm. B. γ-Actin or WAVE2 immunofluorescence images of A549 cells with down-regulated β- or γ-actins. Scale bars represent 10 μm. C. WB analysis of A549 cells with down-regulated β- or γ-actins. Graphs represent relative protein levels (Mean ± SD). D. p34-Arc immunoprecipitation analysis of A549 cells with down-regulated β- or &gamma-actins. E. WAVE2 immunoprecipitation analysis of A549 cells with down-regulated β- or γ-actins. F. LSM of A549 cells with down-regulated β-actin with p34-Arc (green, upper panel), WAVE2 (green, lower panel), β-actin or γ-actin immunofluorescent staining. Scale bars represent 2 μm. Asterisk marks the cell with silenced β-actin.From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.Oncotarget. 2015 Jun 10;6:14556-71.
  • WB - Anti Human Actin Gamma Antibody, clone 2A3  ABD12781
    Western blot analysis of NIH 3T3 murine embryonic fibroblast whole cell lysate probed with Mouse anti Human actin gamma antibody followed by HRP conjugated Goat anti Nouse IgG, visualized by chemiluminescence
  • IF - Anti Human Actin Gamma Antibody, clone 2A3  ABD12781
    Published customer image:Mouse anti Human Actin gamma antibody, clone 2A3 used for the evaluation of γ-actin expression in cell lines by immunofluorescence and western blotting.Image caption:β- and γ-actins in neoplastic cells phenotype A. WB analysis of A549 cells (left panel) and HCT116 cells (right panel) with exogenous expression of β- or γ-actins and corresponding shRNAs. Graphs represent relative actins expression (Mean ± SD). B. Semi-quantitative RT-PCR analysis of β- or γ-actins expression in A549 (left panel) and HCT116 (right panel) with corresponding shRNAs. Graphs represent folds of RNA expression in comparison to control cells (Mean ± SD). C. Immunofluorescent staining of A549 cells (left panel) and HCT116 cells (right panel) with exogenous expression of β- or γ-actins and corresponding shRNAs. Scale bars represent 10 μm.From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.Oncotarget. 2015 Jun 10;6:14556-71.
  • IF - Anti Human Actin Gamma Antibody, clone 2A3  ABD12781
    Published customer image:Mouse anti Human Actin gamma antibody, clone 2A3 used for the evaluation of γ-actin expression in A549 cells by immunofluorescence and western blotting.Image caption:γ-actin co-localizes with cofilin1 and regulates it A. LSM of A549 cells with down-regulated β- or γ-actins with β-actin , γ-actin , cofilin1 or DAPI immunofluorescent staining. Scale bars represent 10 μm. B. WB analysis of A549 cells with down-regulated β- or γ-actins. Graphs represent relative protein levels (Mean ± SD). C. Cofilin1 immunoprecipitation analysis of A549 cells with down-regulated β- or γ-actins. D. Cofilin1/γ-actin PLA analysis of A549 cells with down-regulated β- or γ-actins. Scale bar represents 10 μm. Graph represents relative fluorescence intensity (Mean ± SD).From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.Oncotarget. 2015 Jun 10;6:14556-71.
  • WB - Anti Human Actin Gamma Antibody, clone 2A3  ABD12781
    Published customer image:Mouse anti Human Actin gamma antibody, clone 2A3 used for the evaluation of γ-actin expression in A549 cells by immunofluorescence and western blotting.Image caption:γ-actin and ERK1/2 are mutually regulated A. WB analysis of A549 cells with exogenous expression of β- or γ-actins and corresponding shRNAs. Graphs represent relative pERK1/2 expression (Mean ± SD). B. LSM of A549 cells with down-regulated β- or γ-actins with β-actin , γ-actin or pERK1/2 immunofluorescent staining. Scale bars represent 10 μm. C. pERK1/2 immunoprecipitation analysis of A549 cells with down-regulated β- or γ-actins. D. pERK1/2/γ-actin PLA analysis of A549 cells with down-regulated β- or γ-actins. Scale bar represents 10 μm. Graph represents relative fluorescence intensity (Mean ± SD). E. LSM of A549 cells treated with EGF or UO126 with β-actin or γ-actin immunofluorescent staining. Scale bars represent 10 μm. F. WB analysis of A549 cells treated with EGF or UO126. Graphs represent relative pERK1/2 or β-/γ-actin levels (Mean ± SD).From: Dugina V, Khromova N, Rybko V, Blizniukov O, Shagieva G, Chaponnier C, Kopnin B, Kopnin P. Tumor promotion by γ and suppression by β non-muscle actin isoforms.Oncotarget. 2015 Jun 10;6:14556-71.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF
Primary Accession P63261
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Clone Names 2A3
Calculated MW 41793 Da
Additional Information
Other Species Mammalian species,M
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Synthetic peptide corresponding to the N-terminal nonapeptide of gamma-cytoplasmic actin, coupled to KLH.
Shelf Life 18 months from date of despatch.
Gene ID 71
Other Names Actin, cytoplasmic 2, Gamma-actin, Actin, cytoplasmic 2, N-terminally processed, ACTG1, ACTG
Target/Specificity Mouse anti Human actin gamma antibody, clone 2A3 recognises cytoplasmic actin gamma (known also as ACTG and Gamma Actin), a 41.8 kDa cytoskeletal protein. Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified (Vandekerckhove and Weber 1978) . Clone 2A3 is highly specific for actin gamma and does not react with other actin isoforms (Duginaet al.2009).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human Actin Gamma Antibody, clone 2A3 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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