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>   home   >   Products   >   Primary Antibodies   >   Anti Staphylococcus aureus ATCC 29740 Antibody, clone Staph11-232.3    

Anti Staphylococcus aureus ATCC 29740 Antibody, clone Staph11-232.3

Mouse Anti-Bacterial Monoclonal Antibody

  • E - Anti Staphylococcus aureus ATCC 29740 Antibody, clone Staph11-232.3  ABD12796
    Published customer image:Mouse anti Staphylococcus aureus antibody, clone Staph11-232.3 used as a capture reagent in an ELISA to evaluate linear peptide binding.Image caption:Both SP27 and MAP27' bind to anti-PGN mAb specifically. (A) The sequences of SP27 and SP27' . Underlined letters represent nonspecific flanking amino acids. (B) Both SP27 and SP27' bind to anti-PGN mAb in a dose-dependent manner. (C) Both SP27 and SP27' (100 µg/ml) specifically bind to anti-PGN mAb, but not to anti-LTA mAb. For ELISA assays in panel B and C, anti-PGN mAb or anti-LTA mAb was used to coat the wells at a concentration of 5 µg/ml, SP27, SP27' or non-specific peptide L2 (in panel B, sequence: Biotin-HSGHWDFRQWWQPSGG) was then added at indicated concentrations and incubated at 37°C for 40 min, followed by detection with HRP-labeled streptavidin. (D) The structure diagram of MAP27. MAP27 was synthesized in a tetra-branched form that contains four copies of a sequence that mimics PGN epitope. (E) MAP27 binds to anti-PGN mAb specifically. (F) MAP27 binds to anti-S. aureus polyclonal antibodies specifically. For ELISA assays in panel E and F, MAP27 or MAPctrl was used to coat the wells of a microplate. Anti-PGN mAb or anti-S. aureus polyclonal antibodies was added, followed by detection with HRP-labeled antibodies. The absorbance was measured at OD450nm. The results are shown as means ±SEM. * P
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Bacteria
Host Mouse
Clonality Monoclonal
Isotype IgG3
Clone Names Staph11-232.3
Additional Information
Purification Purified IgG3 prepared by affinity chromatography from ascites
Immunogen UV-inactivatedStaphylococcus aureus cells (ATCC 29740).
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti Staphylococcus aureus ATCC 29740 antibody, clone Staph11-232.3 recognizes the peptidoglycan of S. aureus, protein A-negative S. aureusandS. epidermidis.Mouse anti Staphylococcus aureus ATCC 29740 antibody, clone Staph11-232.3 does not cross-react with Streptococcus sp., Mycoplasma sp. or Cornybacteria sp., however it has not been cross-adsorbed and may react with peptidoglycans from other related species.
Preservative & Stabilisers 0.1% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Staphylococcus aureus ATCC 29740 Antibody, clone Staph11-232.3 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12796
(40 western blots)
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