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Anti Rat CD1d Antibody, clone WTH1

Mouse Anti-Rat Monoclonal Antibody

  • FC - Anti Rat CD1d Antibody, clone WTH1  ABD12798
    Staining of rat thymocytes with Mouse anti Rat CD1d:FITC
  • IHC - Anti Rat CD1d Antibody, clone WTH1  ABD12798
    Immunoperoxidase staining of LEW rat spleen follicle with Mouse anti Rat CD1d
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-F, FC, E, IP, Func
Primary Accession P11609
Other Accession Q63493
Reactivity Rat
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names WTH1
Calculated MW 38554 Da
Additional Information
Other Species M
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Rat CD1d-transduced M12.4.1.C3 cells.
Shelf Life 18 months from date of despatch.
Gene ID 12479
Other Names Antigen-presenting glycoprotein CD1d1, CD1d.1, Cd1d1, Cd1.1
Target/Specificity Mouse anti Rat CD1d antibody, clone WTH1 recognizes rat CD1d, a glycoprotein expressed on cortical thymocytes, certain T-cell leukaemias and various other antigen presenting cells. CD1d binds self and non-self glycolipids and presents them to T-cell receptors on natural killer cells. When activated, natural killer cells produce Th1 and Th2 cytokines.Clone WTH1 recognizes a different, non-overlapping epitope to clone WTH2 and binds with high affinity to the mature CD1d heavy chain independently of beta-2-microglobulin. Mouse anti Rat CD1d antibody, clone WTH1 efficiently blocks rat and mouse invariant natural killer T cell (iNKT cell) response to a-GalCer (an antigen for iNKT cells) and has a slightly higher affinity to native CD1d than clone WTH2.Clone WTH1 cross reacts weakly with mouse tissue in IHC and western blotting, but reacts strongly with both rat and mouse antigens in flow cytometry and immunoprecipitation.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Rat CD1d Antibody, clone WTH1 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12798
(40 western blots)
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