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Mouse Anti-Rat Monoclonal Antibody
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- CITATIONS
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- BACKGROUND
Application 
| WB, IHC-F, FC, E, IP, Func |
|---|---|
| Primary Accession | Q63493 |
| Other Accession | P11609 |
| Reactivity | Rat |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone Names | WTH2 |
| Calculated MW | 38542 Da |
| Other Species | M |
|---|---|
| Immunogen | Rat CD1d-transduced M12.4.1.C3 cells. |
| Shelf Life | 18 months from date of despatch. |
| Gene ID | 25109 |
| Other Names | Antigen-presenting glycoprotein CD1d, CD1d, Cd1d, Cd1d1 |
| Target/Specificity | Mouse anti Rat CD1d antibody, clone WTH2 recognizes rat CD1d, a glycoprotein expressed on cortical thymocytes, certain T-cell leukaemias and various other antigen presenting cells. CD1d binds self and non-self glycolipids and presents them to T-cell receptors on natural killer cells. When activated, natural killer cells produce Th1 and Th2 cytokines.Clone WTH2 recognizes a different, non-overlapping epitope than clone WTH1 and binds with high affinity to the mature CD1d heavy chain independently of beta-2-microglobulin. Mouse anti Rat CD1d antibody, clone WTH2 efficiently blocks rat and mouse invariant natural killer T cell (iNKT cell) response to a-GalCer (an antigen for iNKT cells), consistently blocks activation of mouse type II NKT cell hybridomas and has a slightly lower affinity to native CD1d than clone WTH1.In western blotting clone WTH2 reacts weakly with rat tissue and does not cross react with mouse tissue. It reacts strongly with both rat and mouse antigens in flow cytometry and cross reacts weakly with mouse tissue in IHC and immunoprecipitation. |
| Preservative & Stabilisers | 0.09% Sodium Azide (NaN3) |
| Storage | Store at +4℃ or at -20℃ if preferred. |
| Precautions | Anti Rat CD1d Antibody, clone WTH2 is for research use only and not for use in diagnostic or therapeutic procedures. |
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Application Protocols
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Cat# ABD12799
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