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Anti Rat CD1d Antibody, clone WTH2

Mouse Anti-Rat Monoclonal Antibody

  • FC - Anti Rat CD1d Antibody, clone WTH2  ABD12799
    Staining of rat thymocytes with Mouse anti Rat CD1d
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-F, FC, E, IP, Func
Primary Accession Q63493
Other Accession P11609
Reactivity Rat
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names WTH2
Calculated MW 38542 Da
Additional Information
Other Species M
Immunogen Rat CD1d-transduced M12.4.1.C3 cells.
Shelf Life 18 months from date of despatch.
Gene ID 25109
Other Names Antigen-presenting glycoprotein CD1d, CD1d, Cd1d, Cd1d1
Target/Specificity Mouse anti Rat CD1d antibody, clone WTH2 recognizes rat CD1d, a glycoprotein expressed on cortical thymocytes, certain T-cell leukaemias and various other antigen presenting cells. CD1d binds self and non-self glycolipids and presents them to T-cell receptors on natural killer cells. When activated, natural killer cells produce Th1 and Th2 cytokines.Clone WTH2 recognizes a different, non-overlapping epitope than clone WTH1 and binds with high affinity to the mature CD1d heavy chain independently of beta-2-microglobulin. Mouse anti Rat CD1d antibody, clone WTH2 efficiently blocks rat and mouse invariant natural killer T cell (iNKT cell) response to a-GalCer (an antigen for iNKT cells), consistently blocks activation of mouse type II NKT cell hybridomas and has a slightly lower affinity to native CD1d than clone WTH1.In western blotting clone WTH2 reacts weakly with rat tissue and does not cross react with mouse tissue. It reacts strongly with both rat and mouse antigens in flow cytometry and cross reacts weakly with mouse tissue in IHC and immunoprecipitation.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Rat CD1d Antibody, clone WTH2 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12799
(40 western blots)
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