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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti Dog CD25 Antibody, clone P4A10    

Anti Dog CD25 Antibody, clone P4A10

Mouse Anti-Dog Monoclonal Antibody

  • FC - Anti Dog CD25 Antibody, clone P4A10  ABD12804
    Canine peripheral blood lymphocytes stained with Mouse anti Dog CD25:FITC and dual stained with Rat anti Dog CD4:Alexa Fluor®647
  • FC - Anti Dog CD25 Antibody, clone P4A10  ABD12804
    Canine peripheral blood lymphocytes stained with Mouse anti Dog CD25:RPE and dual stained with Rat anti Dog CD4:Alexa Fluor®647
  • FC - Anti Dog CD25 Antibody, clone P4A10  ABD12804
    Canine peripheral blood lymphocytes stained with Mouse anti Dog CD25 followed by Goat anti Mouse IgG:FITC and dual stained with Rat anti Dog CD4:Alexa Fluor®647
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O62802
Reactivity Dog
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names P4A10
Calculated MW 30357 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Enriched and stimulated Canine T cells.
Shelf Life 18 months from date of despatch.
Gene ID 403870
Other Names Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, CD25, IL2RA
Target/Specificity Mouse anti Dog CD25, clone P4A10 recognises the canine homologue of the human CD25 cell surface antigen, also known at IL-2Rα, a glycoprotein of approximately 55 kDa expressed primarily by activated T lymphocytes (Abramset al.2010). The IL-2 receptor is composed of 3 subunits, an α chain (CD25), a β chain (CD122) and a γ chain (CD132), CD25 functions as a low affinity receptor for IL-2.Research has demonstrated that antibodies to CD4, FoxP3 and CD25 may be used in studies of T regulatory cells (Tregs), a unique subset of T helper cells that function in the control of effector cells vital in preventing autoimmunity. Previous studies of canine Tregs have utilized the mouse anti human CD25 monoclonal antibody, clone ACT1, which has demonstrated cross reactivity to canine CD25 (Rissettoet al.2010). However this clone has poor affinity for canine CD25. The development of clone P4A10 now offers a specific monoclonal Mouse anti Canine CD25, demonstrating a greater affinity for canine CD25 than the cross reactive anti human antibody (Abramset al.2010).The dog is an important veterinary species in its own right. In addition dogs are used as an animal model in the study of a number of serious human disease states including various forms cancers (Paoloniet al.2008), and in genetically related diseases of the hemopoitic system (Baueret al.2009). The generation of clone P4A10 provides an improved tool for the identification of canine CD25 and will facilitate studies in this species.
Preservative & Stabilisers 0.09% Sodium Azide, 1% Bovine Serum Albumin
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Dog CD25 Antibody, clone P4A10 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12804
(40 western blots)
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