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Anti Chicken CD14 Antibody, clone AV141

Mouse Anti-Chicken Monoclonal Antibody

  • E - Anti Chicken CD14 Antibody, clone AV141  ABD12808
    Indirect ELISA using clone AV141.Plates were coated with CD14/Ig fusion protein , control/Ig fusion protein was used as a negative control, CD14/V5H6 fusion protein (COS s/n), mock transfected COS s/n was used as a second negative control. The antibody was used at 4ug/ml and then 1/3 dilutions. The secondary antibody used was goat anti-mouse IgG absorbed against human (Image courtesy of the Roslin Institute.)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Chicken
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names AV141
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Chicken CD14/huIg fusion protein.
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti Chicken CD14 antibody, clone AV141 recognises chicken monocyte differentiation antigen CD14, a 465 amino acid GPI-anchored membrane glycoprotein containing multiple CD14 is a monocyte/macrophage differentiation antigen, belonging to the lipopolysaccharide receptor family, also expressed weakly on microglia and Langerhans cells.CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation and the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules.Clone AV141 is one of a range of antibodies that have been developed by the Roslin Institute as part of the Avian immulogy toolbox project. In addition to Mouse anti Chicken CD14 antibody, clone AV141, other antibodies to chicken CD antigens and cytokines from this project are now available from AbD Serotec.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Chicken CD14 Antibody, clone AV141 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12808
(40 western blots)
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