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Anti Human CD42a Antibody, clone FMC-25

Mouse Anti-Human Monoclonal Antibody

  • FC - Anti Human CD42a Antibody, clone FMC-25  ABD12815
    Human peripheral blood platelets stained with Mouse anti Human CD42a:FITC
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P14770
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names FMC-25
Calculated MW 19046 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Peripheral blood mononuclear cells.
Shelf Life 18 months from the date of despatch
Gene ID 2815
Other Names Platelet glycoprotein IX, GP-IX, GPIX, Glycoprotein 9, CD42a, GP9
Target/Specificity Mouse anti Human CD42a antibody, clone FMC-25 recognizes human CD42a, also known as Platelet glycoprotein IX, Glycoprotein 9 or GP-IX. CD42a is a 177 amino acid, ~20kDa type I single pass transmembrane glycoprotein containing a single leucine-rich repeat containing N-terminal domain and a single leucine-rich repeat containing C-terminaldomain.CD42a is expressed by platelets and megakaryocytes and forms a covalent complex with CD42c (GP-1b-beta), CD42b (GP-1b-alpha) and CD42d (platelet glycoprotein V) to create the platelet surface receptor for von Willebrand factor. Incubation of the intact von Willebrand receptor complex with clone FMC-25 does not appear to inhibit binding of von Willebrand factor to the receptor (Yanet al.2011). Defects in the GP1BB gene encoding human CD42a can lead to the inherited bleeding disorder Bernard-Soulier syndrome (Diz-Küçükkaya 2013), characterized by prolonged bleeding times, thrombocytopenia and the appearence of giant platelets in the circulation (Johnset al.2014).Mouse anti human CD42a antibody, clone FMC-25 has been successfully used as a capture reagent for platelet-autoantibody complexes in the sera of patients presenting thrombocytopenia associated with anti-phospholipid syndrome (Godeauet al.1997).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human CD42a Antibody, clone FMC-25 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12815
(40 western blots)
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