|Application ||WB, IHC-P, IF, E, IP|
|Calculated MW||207721 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||E.coli expressed BRCA1 protein fragment corresponding to amino acids 762-1315|
|Shelf Life||18 months from date of despatch.|
|Other Names||Breast cancer type 1 susceptibility protein, 6.3.2.-, RING finger protein 53, BRCA1, RNF53|
|Target/Specificity||Mouse anti BRCA1, clone 17F8 recognises both human and mouse BRCA1, otherwise known as breast cancer type 1 susceptibility protein, a tumor suppressor gene and major player in DNA damage repair, predominantly expressed in the nucleus during the S/G2 phase of the cell cycle.Along with BRCA2, BRCA1 is a high risk gene which is associated with hereditary breast and ovarian cancers, particularly at a younger age of diagnosis. Women carrying the BRCA1 mutation have a 50-95% chance of developing breast cancer in later life, but genetic screening and increased awareness of preventative surgery, can reduce this risk significantly. Deleterious BRCA1 mutations may also increase the risk of other cancers in both males and females including pancreatic cancer, although in males pancreatic and prostate cancer appear to be more strongly associated with BRCA2 gene mutations.BRCA1 is a key marker of triple-negative breast cancer/TNBC (ER-/PR-/HER2-), a high risk aggressive cancer which makes up about 15% of invasive breast cancers, and which lacks the benefit of specific therapy that targets the three major proteins ER/PR/HER2. Triple-negative tumors are predominantly basal-like, poorly differentiated and of higher histological grade. Younger women have an increased rate of basal or BRCA related TNBC, compared with the higher proportion of apocrine, normal-like and rare subtypes of TNBC, seen in older women.Mouse anti BRCA1, clone17F8 recognises full length 220kDa BRCA1, but not the delta exon 11 splice variant. Mouse anti BRCA1, clone17F8 is well documented for use in immunohistochemistry, and studies using immunofluorescence staining, have shown that this antibody labels both cytoplasmic BRCA1 and the centromeric region (overlapping with the nucleus), and exhibits cytoplasmic staining of BRCA1 in breast cancer cell lines and nuclear staining in several non-breast cancer cell lines (Lee,et al.1998).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20℃ if preferred.|
|Precautions||Anti BRCA1 Antibody, clone 17F8 is for research use only and not for use in diagnostic or therapeutic procedures.|
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