|Application ||WB, IHC-P, IHC-F, IP|
|Calculated MW||207721 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||E.coli expressed BRCA1 protein fragment corresponding to amino acids 341-748|
|Shelf Life||18 months from date of despatch.|
|Other Names||Breast cancer type 1 susceptibility protein, 6.3.2.-, RING finger protein 53, BRCA1, RNF53|
|Target/Specificity||Mouse anti Human BRCA1, clone 8F7 recognises human BRCA1, otherwise known as breast cancer type 1 susceptibility protein, a tumor suppressor gene and major player in DNA damage repair, predominantly expressed in the nucleus during the S/G2 phase of the cell cycle. Along with BRCA2, BRCA1 is a high risk gene which is associated with hereditary breast and ovarian cancers, particularly at a younger age of diagnosis. Women carrying the BRCA1 mutation have a 50-95% chance of developing breast cancer in later life, but genetic screening and increased awareness of preventative surgery, can reduce this risk significantly.Deleterious BRCA1 mutations may also increase the risk of other cancers in both males and females including pancreatic cancer, although in males pancreatic and prostate cancer appear to be more strongly associated with BRCA2 gene mutations.BRCA1 is a key marker of triple-negative breast cancer/TNBC (ER-/PR-/HER2-), a high risk aggressive cancer which makes up about 15% of invasive breast cancers, and which lacks the benefit of specific therapy that targets the three major proteins ER/PR/HER2. Triple-negative tumors are predominantly basal-like, poorly differentiated and of higher histological grade. Younger women have an increased rate of basal or BRCA related TNBC, compared with the higher proportion of apocrine, normal-like and rare subtypes of TNBC, seen in older women. Mouse anti BRCA1, clone 8F7 recognises full length 220kDa BRCA1, and is suitable for use in immunohistochemical studies of both frozen and paraffin sections see Yoshikawa, K. et al. for details. . Several studies have shown that clone 8F7 exhibits strong and highly specific nuclear staining.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20℃ if preferred.|
|Precautions||Anti Human BRCA1 Antibody, clone 8F7 is for research use only and not for use in diagnostic or therapeutic procedures.|
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