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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti Mouse CD8 Alpha Antibody, clone KT15    

Anti Mouse CD8 Alpha Antibody, clone KT15

Rat Anti-Mouse Monoclonal Antibody

  • IF - Anti Mouse CD8 Alpha Antibody, clone KT15  ABD12822
    Published customer image:Rat anti Mouse CD8α antibody, clone KT15 use for the detection of CD8 positive T cells from tumors by flow cytometry and immunofluorescence.Image caption:Vaccination with pFap suppresses TAM, MDSC, Treg, and enhances DC and CTL recruitment.Primary tumors were isolated from mice 25 days after orthotopic challenge and immune cells were identified in primary tumors using antibodies for the following: (A) tumor-associated macrophages : F4/80 , (B) myeloid derived suppressor cells : CD11b (red, nuclear) and Gr-1 (green, nuclear), (C) T regulatory cells (Tregs, white arrowheads): CD4 (red, cell surface) and FOXP3 (green, nuclear), (D) dendritic cells : 33D1 , and (E) cytotoxic T lymphocytes : CD8 Scale bar for all panels=100 µm.From: Liao D, Luo Y, Markowitz D, Xiang R, Reisfeld RA Cancer Associated Fibroblasts Promote Tumor Growth and Metastasis by Modulating the Tumor Immune Microenvironment in a 4T1 Murine Breast Cancer Model. PLoS ONE 4: e7965.
  • FC - Anti Mouse CD8 Alpha Antibody, clone KT15  ABD12822
    Published customer image:Rat anti Mouse CD8α antibody, clone KT15 used for the detection of CD8 positive lymphocytes by flow cytometry.Image caption:Vaccination with xIM-1.6.a induces CD4 and CD8 T cells memory response. Mice were immunized and aerosol challenged as mentioned in Figure 1. Pooled lymphocytes from spleen and lymphnodes of immunized animals were stimulated in vitro with PPD (50 µg/ml) for 48 h and later stained with fluorochrome tagged antibodies and analyzed by flowcytometry. A, flow contours depict percentage of CD4 T cells expressing IL-7R and CD44 (upper panel); CD62L expression on CD4 T cells (lower panel). C, CD8 T cells expressing IL-7R and CD44 (upper panel); CD62L on CD8 T cells (lower panel). Bar diagrams show total number of CD4 (B) and CD8 (D) T cells expressing CD44hi, CD62Lhi and CD62Llo. Data shown are mean ± SEM of two independent experiments with n = 3 animals per group. ‘*’ indicates p
  • FC - Anti Mouse CD8 Alpha Antibody, clone KT15  ABD12822
    Staining of mouse spleen cells with Rat anti Mouse CD8
  • IHC - Anti Mouse CD8 Alpha Antibody, clone KT15  ABD12822
    Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8α antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG s a detection reagent. Low power
  • FC - Anti Mouse CD8 Alpha Antibody, clone KT15  ABD12822
    Staining of mouse spleen with Rat anti Mouse CD8 Alpha: RPE
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P01731
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names KT15
Calculated MW 27456 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen T cell clone, C6.
Shelf Life 18 months from date of despatch.
Gene ID 12525
Other Names T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, CD8a, Cd8a, Lyt-2, Lyt2
Target/Specificity Rat anti mouse CD8α, clone KT15, recognizes the alpha chain of mouse CD8. CD8 is a heterodimeric protein composed of disulphide-linked CD8α and CD8β chains that is expressed primarily on cytotoxic T-cells. CD8 functions in the interaction with MHC Class I-bearing targets and plays a role in T-cell-mediated killing (Nakauchi , H. et al., 1985&Nakauchi, H. et al., 1987).Clone KT15 is reported to block T-cell-mediated cytotoxicity in in vitro assays (Zeis, M. et al., 2002).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3),Sucrose
Storage Store at +4℃. DO NOT FREEZE.
PrecautionsAnti Mouse CD8 Alpha Antibody, clone KT15 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12822
(40 western blots)
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