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>   home   >   Products   >   Primary Antibodies   >   Anti Rat CD11b Antibody, clone ED8    

Anti Rat CD11b Antibody, clone ED8

Mouse Anti-Rat Monoclonal Antibody

     
  • IF - Anti Rat CD11b Antibody, clone ED8  ABD12826
    Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED8 , red in A and CD4 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power
  • FC - Anti Rat CD11b Antibody, clone ED8  ABD12826
    Published customer image:Lithium inhibits lipopolysaccharide -induced microglial activation. Primary microglial cells were pretreated with LiCl at 0.3, 1 and 3 mM for 30 minutes and then stimulated with LPS at 10 ng/ml for 24 hours. (a) and (b) For flow cytometric analysis, the cells were incubated with PE-conjugated ED8 antibody at 37°C for 1 hour. **P
  • IHC - Anti Rat CD11b Antibody, clone ED8  ABD12826
    Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11 antibody, clone ED8 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power
  • IF - Anti Rat CD11b Antibody, clone ED8  ABD12826
    Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED8 , red in A and CD4 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Reactivity Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names ED8
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Rat spleen cell homogenate with Freund's complete adjuvant.
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti Rat CD11b antibody, clone ED8 recognizes a membrane antigen on rat macrophages, monocytes, dendritic cells and granulocytes. It also recognizes small ramified microglia in the central nervous system. No other cell types are positive for ED8. The recognized antigen is a heterodimer (160 and 95 kD) belonging to the family of adhesion molecules CD11b/CD18, also designated as Mac-1 antigen or CR3. Mouse anti Rat CD11b antibody, clones ED7 and ED8 may recognize closely related epitopes on the same molecule. ED7 and ED8 have been shown to induce homotypic aggregation of granulocytes.
Preservative & Stabilisers 0.09% Sodium Azide, 1% Bovine Serum Albumin
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Rat CD11b Antibody, clone ED8 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12826
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