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>   home   >   Products   >   Primary Antibodies   >   Anti Rat CD172a Antibody, clone ED9    

Anti Rat CD172a Antibody, clone ED9

Mouse Anti-Rat Monoclonal Antibody

     
  • FC - Anti Rat CD172a Antibody, clone ED9  ABD12827
    Staining of rat peritoneal macrophages with Mouse anti Rat CD172a:RPE
  • FC - Anti Rat CD172a Antibody, clone ED9  ABD12827
    Published clone specific image:Flow cytometric analysis of ED7 and ED9 expression of AM following magnetic bead separation. AM of 3 days NO2-exposed rats were separated due to their expression of the cell surface molecule ED7 using magnetic bead separation. Susbsequently, ED7 and ED9 expression was analyzed in unseparated AM (A), ED7-positive AM (B), and ED7-negative AM (C). Numbers right of each histogram represent the mean fluorescence of the respective cell population. The figure clearly demonstrates that ED7-positive AM show a lower ED9 expression compared to ED7-negative AM. Shown is a representative data set of more than twenty animals.From: Garn H, Siese A, Stumpf S, Wensing A, Renz H, Gemsa D. Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats. Respir Res. 2006 Jan 6;7:4.
  • IHC - Anti Rat CD172a Antibody, clone ED9  ABD12827
    Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power
  • IHC - Anti Rat CD172a Antibody, clone ED9  ABD12827
    Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power
  • IF - Anti Rat CD172a Antibody, clone ED9  ABD12827
    Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Primary Accession P97710
Reactivity Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names ED9
Calculated MW 55691 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Spleen cell homogenate.
Shelf Life 18 months from date of despatch.
Gene ID 25528
Other Names Tyrosine-protein phosphatase non-receptor type substrate 1, SHP substrate 1, SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Bit, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, Macrophage fusion receptor, Macrophage membrane protein MFP150, Signal-regulatory protein alpha-1, Sirp-alpha-1, CD172a, Sirpa, Bit, Mfr, Ptpns1, Shps1, Sirp
Target/Specificity Mouse anti Rat CD172a antibody, clone ED9 recognizes rat Tyrosine-protein phosphatase non-receptor type substrate 1, also known as CD172a, Signal-regulatory protein alpha-1, SIRP&alpha, -1, SHP substrate 1, Macrophage membrane protein MFP150 or Macrophage fusion receptor. CD172a is a 509 amino acid ~56 kDa single pass type 1 transmembrane glycoprotein expressed selectively by myeloid cells and by neurons (UniProt: P97710). Mouse anti Rat CD172a antibody, clone ED9 has been reported to bind to an alternative epitpe to another anti CD172 antibody, clone OX-41 (Adamset al.1998) and has been reported to block the interaction of CD172a - CD47 (de Vries et al.2002).
Preservative & Stabilisers 0.09% Sodium Azide, 1% Bovine Serum Albumin
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Rat CD172a Antibody, clone ED9 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12827
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