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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti Mouse CD11b Antibody, clone 5C6    

Anti Mouse CD11b Antibody, clone 5C6

Rat Anti-Mouse Monoclonal Antibody

     
  • IF - Anti Mouse CD11b Antibody, clone 5C6  ABD12843
    Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD11b, clone 5C6 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power
  • IF - Anti Mouse CD11b Antibody, clone 5C6  ABD12843
    Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD11b, clone 5C6 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power
  • IF - Anti Mouse CD11b Antibody, clone 5C6  ABD12843
    Published customer imageBM-derived GFP+ single cells and vessel-associated cells express CD11b. Fluorescence microscopy for GFP combined with immunofluorescence detection of (A) CD11b, (B) vWF and (C) CD31, 24 hours after pMCAO. (A) Fluorescence detection of GFP and CD11b showed that most GFP+ cells co-expressed CD11b (yellow cells, indicated by arrows), and intermingled with CD11b+ host cells. Note also that a few GFP+ cells did not co-express CD11b (arrow head). Insert shows high magnification of GFP+ cells, some of which co-express CD11b, aggregated around a vessel. (B, C) Fluorescence detection of GFP and the endothelial cell markers vWF (B) and CD31 (C). Inserts show higher magnification of sections of the same vessels. Although there are indications that single vWF+ cells co-express GFP (arrows in B), this could not be reproduced using staining for CD31, and the majority of vWF+ and CD31+ cells showed no co-expression of GFP. Instead, GFP remained confined to round and elongated cells located in the juxtavascular space (insert in C). CD11b+ cells were visualized using Alexa Fluor® 568-conjugated goat anti-rat IgG, vWF+ and CD31+ cells using Alexa Fluor® 546-conjugated goat anti-rabbit IgG and Alexa Fluor® 594-conjugated goat anti-rat IgG, respectively. Scale bars: 20 µm (A-C)..From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice.J Neuroinflammation. 2008 Oct 23;5:46.
  • IHC - Anti Mouse CD11b Antibody, clone 5C6  ABD12843
    Immunoperoxidase staining of mouse lymph node cryosection with Rat anti Mouse CD11b antibody, clone 5C6 followed by horseradish peroxidase conjugated Goat anti Rat IgG as a detection reagent. high power
  • FC - Anti Mouse CD11b Antibody, clone 5C6  ABD12843
    Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b:FITC
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-F, IF, FC, IP
Primary Accession P05555
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2b
Clone Names 5C6
Calculated MW 127481 Da
Additional Information
Other Species H
Purification Purified IgG prepared by ion exchange chromatography
Immunogen Thioglycollate-elicted Peritoneal Macrophages (TPM)
Shelf Life 18 months from the date of despatch
Other Names Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, CD11b, Itgam
Target/Specificity Rat anti Mouse CD11b antibody, clone 5C6 recognizes CD11b, also known as the integrin alpha M chain. CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles.Rat anti Mouse CD11b antibody, clone 5C6 immunoprecipitates a heterodimer of ~165 and ~95 kDa.Rat anti Mouse CD11b antibody, clone 5C6 exhibits various functional properties, reportedly inhibiting adhesion in vitro and inflammatory recruitment in vivo. Rat anti Mouse CD11b antibody, clone 5C6 also inhibits delayed hypersensitivity, potentiates bacterial infections and inhibits type 1 diabetes.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Mouse CD11b Antibody, clone 5C6 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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