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>   home   >   Products   >   Primary Antibodies   >   Anti Human HLA DR Antibody, clone YE2/36-HLK    

Anti Human HLA DR Antibody, clone YE2/36-HLK

Rat Anti-Human Monoclonal Antibody

     
  • FC - Anti Human HLA DR Antibody, clone YE2/36-HLK  ABD12846
    Published customer image:Rat anti Human HLA-DR antibody, clone YE2/36-HLK used for flow cytometryImage caption:BILF1 identified as a lytic gene that downregulates surface MHC class I. 293 (A) or MJS (B) cells were transfected with different EBV genes in the bi-cistronic vector, pCDNA3-IRES-nlsGFP. At 48 hr post-transfection, surface MHC class I was stained with PE-conjugated W6/32 mAb and (in MJS only) MHC class II was stained with PE-conjugated anti-DR mAb, YE2/36-HLK. Two-colour flow cytometry was used to analyse staining in the untransfected GFP- population, shown as the solid line histogram, and in the transfected GFP+ population, shown as the dashed line histogram. The grey histogram denotes background staining obtained with an isotype control PE-conjugated antibody.From: Zuo J, Currin A, Griffin BD, Shannon-Lowe C, Thomas WA, et al. The Epstein-Barr Virus G-Protein-Coupled Receptor Contributes to Immune Evasion by Targeting MHC Class I Molecules for Degradation. PLoS Pathog 5(1): e1000255.
  • FC - Anti Human HLA DR Antibody, clone YE2/36-HLK  ABD12846
    Published customer image:Rat anti Human HLA-DR antibody, clone YE2/36-HLK used for flow cytometryImage caption:BILF1 downregulates surface MHC class I expression and inhibits the T cell recognition of endogenous EBV antigen in LCLs. (A) LCLs were transduced with PLZRS-HABILF1-IRES-GFP retrovirus. After 6 days, surface MHC class I was stained with PE-conjugated W6/32 mAb and MHC class II was stained with PE-conjugated anti-DR mAb, YE2/36-HLK. Two-colour flow cytometry was used to analyze staining in the untransduced, GFP-, population, shown as the solid line histogram, and in the transduced GFP+ (BILF1+) population, shown as the dashed line histogram. The grey histogram denotes background staining obtained with an isotype control PE-conjugated antibody. (B) LCL cultures transduced with control retrovirus or with the BILF1 retrovirus were sorted by flow cytometry to generate GFP+/BILF1- and GFP+/BILF1+ lines to use as targets in assays with EBV-specific T cells. The control and BILF1+ LCL targets were incubated with HLA-matched CD8+ effector T cells clones specific for EBNA1 , EBNA3A , or LMP2A peptides, or a CD4+ effector T cell clone specific for a BHRF1 peptide. After 18 hrs the supernatants were tested for the release of IFN-? as a measure of T cell recognition. All results are expressed as IFN-? release in pg/ml and error bars indicate standard deviation of triplicate cultures.From: Zuo J, Currin A, Griffin BD, Shannon-Lowe C, Thomas WA, et al. The Epstein-Barr Virus G-Protein-Coupled Receptor Contributes to Immune Evasion by Targeting MHC Class I Molecules for Degradation. PLoS Pathog 5(1): e1000255.
  • FC - Anti Human HLA DR Antibody, clone YE2/36-HLK  ABD12846
    Published customer image:Rat anti Human HLA-DR antibody, clone YE2/36-HLK used for flow cytometryImage caption:HLA-DR positive monocytes. The percentage HLA-DR positive monocytes was decreased in all patients compared to controls (P = 0.002). The pre-operative ("black square") lowest percentage was seen in patients who developed respiratory failure (P = 0.002). Eighteen hours after intramedullary nailing ("open triangle"), a further decrease in HLA-DR positive monocytes was seen in patients with isolated femur fracture (P < 0.001) and multitrauma patients (P = 0.047).From: Hietbrink, Falco, Leo Koenderman, and Luke PH Leenen. “Intramedullary Nailing of the Femur and the Systemic Activation of Monocytes and Neutrophils. World Journal of Emergency Surgery?: WJES 6 : 34. PMC. Web. 22 Jan. 2015.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Reactivity Human
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names YE2/36-HLK
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen EHR-B Ramos cells.
Shelf Life 18 months from date of despatch.
Target/Specificity Rat anti Human HLA DR antibody, clone YE2/36-HLK recognizes a monomorphic determinant of human HLA DR which is an MHC class II cell surface receptor expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages and activated T lymphocytes.The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class II proteins encoded by the HLA which are HLA DP, HLA DQ and HLA DR.Rat anti Human HLA DR antibody, clone YE2/36-HLK has also been reported to be cross reactive with mouse H-2 haplotypes b, d and q expressed by mouse strains including C57BL/6, DBA, and NZB (Brickell, P.M. et al.1981).
Preservative & Stabilisers 0.09% Sodium Azide, 1% Bovine Serum Albumin
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human HLA DR Antibody, clone YE2/36-HLK is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12846
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