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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti Human CD53 Antibody, clone MEM-53    

Anti Human CD53 Antibody, clone MEM-53

Mouse Anti-Human Monoclonal Antibody

     
  • FC - Anti Human CD53 Antibody, clone MEM-53  ABD12848
    Staining of human peripheral blood monocytes with Mouse anti Human CD53:FITC
  • FC - Anti Human CD53 Antibody, clone MEM-53  ABD12848
    Published customer image:Mouse anti Human CD53 antibody, clone MEM-53 used for the evaluation of CD53 expression on erythroid cultures by flow cytometry.Image caption:Erythroid culture characterisation and expression of tetraspanins and integrins during terminal maturation. A. Temporal expression of erythroid-specific markers, Kell, GPA and AE1 and morphology of the culture at the same time points. AE1 was tested from day 5 onwards. B. Tetraspanin and integrin profile of the same cultures as shown in A. Results are depicted from one culture where directly conjugated antibodies were used (days 3 and 4) and a second culture with indirectly labelled antibodies (day 5 onwards). The y-axis scale is linear to 350 counts; the x-axis is logarithmic to 104. Images were captured on a Leica DM750 microscope, x20 magnification, using Image-Pro Express 6.0 software.From: Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, Chasis JA, et al. Tetraspanins CD81 and CD82 Facilitate a4ß1-Mediated Adhesion of Human Erythroblasts to Vascular Cell Adhesion Molecule-1.PLoS ONE 8(5): e62654.
  • FC - Anti Human CD53 Antibody, clone MEM-53  ABD12848
    Staining of human peripheral blood granulocytes with Mouse anti Human CD53
  • WB - Anti Human CD53 Antibody, clone MEM-53  ABD12848
    Published customer image:Mouse anti Human CD53 antibody, clone MEM-53 used for the evaluation of CD53 expression on erythroid cultures by immunoprecipitation.Image caption:Several anti-tetraspanin antibodies co-precipitate β1 integrins from HEL cells solubilised in Brij-97. Precipitates were prepared from HEL cells solubilised in different detergents in the presence of Mn2+. CD53, MEM-53; CD63, MEM-259; CD81, 454720; CD82, TS82b; CD151, IIG5a; α4, HP2/1; αL, TS1/22; αIIb, PAB-1. Precipitates were run on 7.5% non-reduced gels.From: Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, Chasis JA, et al. Tetraspanins CD81 and CD82 Facilitate a4ß1-Mediated Adhesion of Human Erythroblasts to Vascular Cell Adhesion Molecule-1.PLoS ONE 8(5): e62654.
  • WB - Anti Human CD53 Antibody, clone MEM-53  ABD12848
    Published customer image:Mouse anti Human CD53 antibody, clone MEM-53 used for the evaluation of CD53 expression on erythroid cultures by immunoprecipitation and western blotting.Image caption:Co-precipitation of ß1 and ß3 integrins by tetraspanins with different cations from HEL cells. Precipitates were prepared from HEL cells solubilised in Brij-97 in the presence of EDTA or cations. ß1 is co-precipitated by CD63, CD81, CD82 and CD151 while ß3 is co-precipitated by all tetraspanins in the presence of Mn2+. CD151 co-precipitates ß1 under all conditions. Integrins were separated on 7.5% gels, tetraspanin controls on 12% gels, both non-reducing conditions. CD53, MEM-53; CD63, MEM-259; CD81, 454720; CD82, TS82b; CD151, IIG5a; a4, HP2/1; aL, TS1/22; aIIb, PAB-1.From: Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, Chasis JA, et al. Tetraspanins CD81 and CD82 Facilitate a4ß1-Mediated Adhesion of Human Erythroblasts to Vascular Cell Adhesion Molecule-1.PLoS ONE 8(5): e62654.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Primary Accession P19397
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names MEM-53
Calculated MW 24341 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Shelf Life 18 months from date of despatch.
Gene ID 963
Other Names Leukocyte surface antigen CD53, Cell surface glycoprotein CD53, Tetraspanin-25, Tspan-25, CD53, CD53, MOX44, TSPAN25
Target/Specificity Mouse anti Human CD53 antibody, clone MEM-53 recognizes the human CD53 cell surface antigen, also known as Tetraspanin-25. CD53 is a 219 amino acid multi pass transmembrane glycoprotein of 32-42 kDa. CD53 is expressed by all leucocytes, but is absent from erythrocytes and platelets.
Preservative & Stabilisers 0.09% Sodium Azide, 1% Bovine Serum Albumin
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human CD53 Antibody, clone MEM-53 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12848
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