|Application ||WB, IHC-F, IF, FC|
|Calculated MW||42436 Da|
|Purification||Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serumPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatantPurified IgG prepared by affinity chromatography on Protein G from tissue cul|
|Immunogen||T blasts from a mixed lymphocyte reaction.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Basigin, Glycoprotein CE9, OX-47 antigen, CD147, Bsg|
|Target/Specificity||Mouse anti Rat CD147 antibody, clone OX-47 recognizes a membrane glycoprotein of ~42-48 kDa expressed at high levels on activated lymphocytes but lower levels on resting cells (Patersonet al.1987). CD147 is also present on some endothelia (Fossumet al.1991). The OX-47 antigen contains two Ig-like domains and an unusual transmembrane domain with a charged residue (Fossumet al.1991).CD147 is abundant on activated lymphocytes: it is the homologue of the chicken blood brain barrier antigen HT7.Mouse anti Rat CD147 antibody, clone OX-47 is suitable for use in Western blotting (Fodaet al.2001).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20℃ if preferred.|
|Precautions||Anti Rat CD147 Antibody, clone OX-47 is for research use only and not for use in diagnostic or therapeutic procedures.|
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