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>   home   >   Products   >   Primary Antibodies   >   Anti Human HLA DR Antibody, clone YD1/63.4.10    

Anti Human HLA DR Antibody, clone YD1/63.4.10

Rat Anti-Human Monoclonal Antibody

  • FC - Anti Human HLA DR Antibody, clone YD1/63.4.10  ABD12852
    Published customer image:Rat anti Human HLA-DR antibody, clone YD1/63.4.10 used for the evaluation of MHC class II expression on human PBMC by flow cytometry.Image caption:Plasmacytoid dendritic cells show weak T cell stimulatory capacity. A: Flow cytometric analysis of human PBMC and DC. pDC are characterized as lineage- CD11c- CD303+ cells (middle panel). Dot plots (right side) exemplarily depicts isolated pDC, percentage indicates purity. Histograms show surface expression of CD40, CD80, CD86, CD58 and HLA-DR on resting and CpG/IL-3-activated pDC and cytokine cocktail activated cDC. Isotype-matched control mAb staining is shown in grey. One representative result of three independent experiments is shown. B: T cell stimulatory capacity of activated pDC and cDC. CD4+ or CD8+ Teff were stimulated with allogeneic pDC or cDC at different DC:Teff ratios. Teff proliferation was determined on day 4 of culture by incorporation of 3H-Tdr and presented as mean ± SD of triplicates. One representative experiment out of 3 is shown. C: Expansion of DC-stimulated Teff. Expansion rates of CD4+ or CD8+ Teff after stimulation with allogeneic cDC (n = 4) or pDC (n = 2), determined at day seven. The figure shows the relative fold expansion of initially stimulated Teff. Bar indicates mean values. D: Cytokine profiles of Teff seven days after stimulation with pDC or cDC. Numbers represent the percentage of cytokine-positive Teff. One typical result out of six is shown. ** p
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Human
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names YD1/63.4.10
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen DAUDI cells.
Shelf Life 18 months from date of despatch.
Target/Specificity Rat anti Human HLA DR antibody, clone YD1/63.4.10 recognizes a monomorphic determinant of human HLA DR which is an MHC class II cell surface receptor expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages and activated T lymphocytes.The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class II proteins encoded by the HLA which are HLA DP, HLA DQ and HLA DR.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human HLA DR Antibody, clone YD1/63.4.10 is for research use only and not for use in diagnostic or therapeutic procedures.
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$ 345.00
Cat# ABD12852
1 Formats Available
Availability: 5-7days
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