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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti Mouse CD11b Antibody, clone M1/70.15    

Anti Mouse CD11b Antibody, clone M1/70.15

Rat Anti-Mouse Monoclonal Antibody

     
  • IHC - Anti Mouse CD11b Antibody, clone M1/70.15  ABD12861
    Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG . Medium power
  • FC - Anti Mouse CD11b Antibody, clone M1/70.15  ABD12861
    Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b: Alexa Fluor® 647
  • IHC - Anti Mouse CD11b Antibody, clone M1/70.15  ABD12861
    Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG . High power
  • FC - Anti Mouse CD11b Antibody, clone M1/70.15  ABD12861
    Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD11b:FITC
  • FC - Anti Mouse CD11b Antibody, clone M1/70.15  ABD12861
    Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b:RPE
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Primary Accession P05555
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2b
Clone Names M1/70.15
Calculated MW 127481 Da
Additional Information
Other Species H,Rb
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen T cell enriched splenocytes from B10 mice
Shelf Life 18 months from date of despatch.
Other Names Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, CD11b, Itgam
Target/Specificity Rat anti Mouse CD11b antibody, clone M1/70.15 recognizes the murine CD11b cell surface antigen also known as the alpha M integrin chain or MAC-1, a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to block iC3b binding to its receptor (Belleret al.1982).Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue (Whitelandet al.1995). This product is routinely tested in flow cytometry on mouse peritoneal macrophages.
Preservative & Stabilisers 0.09% Sodium Azide, 1% Bovine Serum
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Mouse CD11b Antibody, clone M1/70.15 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD12861
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