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>   home   >   Products   >   Primary Antibodies   >   Anti Mouse Ly-6B.2 Alloantigen Antibody, clone 7/4    

Anti Mouse Ly-6B.2 Alloantigen Antibody, clone 7/4

Rat Anti-Mouse Monoclonal Antibody

     
  • IHC - Anti Mouse Ly-6B.2 Alloantigen Antibody, clone 7/4  ABD12869
    Published customer image:Rat anti Mouse Ly-6B.2 antibody, clone 7/4 used for the detection of Ly-6B.2 positive cells by immunohistochemistry.Image caption:Effect of atorvastatin on platelet and neutrophil infiltration after reperfusion. The infiltration of platelets (upper panel) and neutrophils (lower panel) were evaluated by immunohistochemistry in the 4 chimeric groups that received 40 min of LAD occlusion and 60 min of reperfusion. Platelets and neutrophils were found predominantly in the ischemic area in B6/B6 chimeras. Atorvastatin reduced both platelet and neutrophil infiltration in B6/B6 and B6/KO chimeras, but not in KO/B6 chimeras.From: Tian Y, Linden J, French BA, Yang Z Atorvastatin at Reperfusion Reduces Myocardial Infarct Size in Mice by Activating eNOS in Bone Marrow-Derived Cells. PLoS ONE 9: e114375.
  • IHC - Anti Mouse Ly-6B.2 Alloantigen Antibody, clone 7/4  ABD12869
    Published customer image:Rat anti Mouse Ly-6B.2 antibody, clone 7/4 used for the detection of Ly-6B.2 positive cells by immunohistochemistry.Image caption:Renal inflammatory changes in Ntn1+/- mice following ischemia. Ntn1+/- mice and their respective age-, weight-, and gender-matched littermate controls (Ntn1+/+) were subjected to 30 minutes of left renal artery ischemia. (A–D) Neutrophil staining. Arrows indicate neutrophils (magnification 400×). (E) Quantification of neutrophil tissue accumulation by measurement of myeloperoxidase . (F) TNF-a and (G) interleukin-6 (IL-6) and (H) interleukin-10 were assessed by real-time RT-PCR from renal tissues. Data were calculated relative to ß-actin and are expressed as fold change compared to sham-operated animals without ischemia (-I). Data are representative of four to six independent experiments for each experimental condition (mean ± SD).From: Grenz A, Dalton JH, Bauerle JD, Badulak A, Ridyard D, et al. Partial Netrin-1 Deficiency Aggravates Acute Kidney Injury. PLoS ONE 6(5): e14812.
  • IHC - Anti Mouse Ly-6B.2 Alloantigen Antibody, clone 7/4  ABD12869
    Published customer image:Rat anti Mouse Ly-6B.2 antibody, clone 7/4 used for the detection of Ly-6B.2 positive cells by immunohistochemistry.Image caption:Peri-incisional infiltration of neutrophils, immunohistochemical appearance. Hind paw skin from incised mice was processed for the identification of neutrophils. Panel A displays an H&E stained section of plantar hind paw skin demonstrating a predominantly dermal cellular infiltrate 2 hours after incision. The dermal and epidermal layers are labeled. Panel B displays the appearance of non-incised hind paw skin stained with a neutrophil-specific antibody. Panel C displays a micrograph taken of incised skin stained for neutrophils 2 hours after incision. Note the abundance of darkly staining infiltrating neutrophils in the dermis of this section compared to that displayed in panel B. The scale bar in panel C is 150 µm in length. All micrographs were taken using 200× magnification.From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.
  • FC - Anti Mouse Ly-6B.2 Alloantigen Antibody, clone 7/4  ABD12869
    Published customer image:Rat anti Mouse Ly-6B.2 monoclonal antibody, clone 7/4 used for the identification of infiltrating neutrophils in murine pulmonary tissue sections by immunohistochemistry.Image caption:Apigenin decreases LPS-induced neutrophil infiltration in the lungs. Lungs from mice receiving Api + LPS, LPS, Api or vehicle were collected at 24 h post-challenge. (A) H&E staining; (B) Chloroacetate esterase staining; (C) Lungs stained with anti-7/4 antibodies; (D) Leukocyte infiltration determined by counting CAE-positive cells per high power field in lungs used in (B); (E) Number of infiltrated neutrophils as determined by counting stained cells per cpf in in lungs used in (C). Data represent the mean ± SEM (N = 9 mice for each biological condition; * p < 0.05 vs. LPS). Scale bars for all figures are 25 μm and 100 μm for insets.From: Cardenas, H.; Arango, D.; Nicholas, C.; Duarte, S.; Nuovo, G.J.; He, W.; Voss, O.H.; Gonzalez-Mejia, M.E.; Guttridge, D.C.; Grotewold, E.; Doseff, A.I.Dietary Apigenin Exerts Immune-Regulatory Activity in Vivo by Reducing NF-κB Activity, Halting Leukocyte Infiltration and Restoring Normal Metabolic Function.Int. J. Mol. Sci. 2016, 17, 323.
  • IHC - Anti Mouse Ly-6B.2 Alloantigen Antibody, clone 7/4  ABD12869
    Published customer image:Rat anti Mouse Ly-6B.2 monoclonal antibody, clone 7/4 used for the detection of neutrophils in murine kidney tissue sections by immunohistochemistry. Macrophages were detected using Rat anti Mouse F4/80 antibody, clone A3-1 Image caption:Immunostaining of macrophages and neutrophils in the kidneys of M-Rac1 FC and KO mice. (A) Representative micrographs of F4/80 immunostaining in the kidneys of Vehicle- or LPS-injected M-Rac1 FC and KO mice. Original magnification x 200. (B) Quantitative analysis of F4/80-positive macrophages. Data are means ± s.e.m. Statistical analysis was performed by two-way ANOVA, n.s. genotype effect, P < 0.01 treatment effect, n.s. interaction effect. **P < 0.01 by Bonferroni's post hoc test. n = 5 per each group. (C) Representative micrographs of Ly-6B.2 (mAb 7/4) immunostaining. (D) Quantitative analysis of Ly-6B.2-positive neutrophils. Statistical analysis was performed by two-way ANOVA, n.s. genotype effect, P < 0.01 treatment effect, n.s. interaction effect. **P < 0.01 by Bonferroni's post hoc test. n = 6 per group.From: Nagase M, Kurihara H, Aiba A, Young MJ, Sakai T Deletion of Rac1GTPase in the Myeloid Lineage Protects against Inflammation-Mediated Kidney Injury in Mice.PLoS ONE 11(3): e0150886.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC
Reactivity Mouse
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names 7/4
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Cultured bone marrow cells
Shelf Life 18 months from date of despatch.
Target/Specificity Rat anti Mouse Ly-6B.2 monoclonal antibody, clone 7/4 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages (Rosaset al.2010). High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa (Rosaset al.2010).In common with other Ly-6 antigens Ly-6B.2 demonstrates a polymorphic expression on inbred mouse strains (Kimuraet al.1984). Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains whilst A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity (Hirsch and Gordon 1982).Rat anti mouse Ly-6B.2 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow (Bertoncelloet al.1991) and the depletion of myeloid cells in vivo(Rosaset al.2010).
Preservative & Stabilisers 0.09% Sodium Azide, 1% Bovine Serum
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Mouse Ly-6B.2 Alloantigen Antibody, clone 7/4 is for research use only and not for use in diagnostic or therapeutic procedures.
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