|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||Cultured bone marrow cells|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Rat anti Mouse Ly-6B.2 monoclonal antibody, clone 7/4 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages (Rosaset al.2010). High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa (Rosaset al.2010).In common with other Ly-6 antigens Ly-6B.2 demonstrates a polymorphic expression on inbred mouse strains (Kimuraet al.1984). Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains whilst A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity (Hirsch and Gordon 1982).Rat anti mouse Ly-6B.2 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow (Bertoncelloet al.1991) and the depletion of myeloid cells in vivo(Rosaset al.2010).|
|Preservative & Stabilisers||0.09% Sodium Azide, 1% Bovine Serum|
|Storage||Store at +4℃ or at -20℃ if preferred.|
|Precautions||Anti Mouse Ly-6B.2 Alloantigen Antibody, clone 7/4 is for research use only and not for use in diagnostic or therapeutic procedures.|
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