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>   home   >   Products   >   Primary Antibodies   >   Anti Tubulin Alpha Antibody, clone YL1/2    

Anti Tubulin Alpha Antibody, clone YL1/2

Rat Anti-Yeast Monoclonal Antibody

  • IF - Anti Tubulin Alpha Antibody, clone YL1/2  ABD12874
    Published customer image:Rat anti tubulin-α antibody, clone YL1/2 used for the detection of tubulin expression in the cnidarian Clytia hemisphaerica by immunofluorescence.Image caption:Distribution of the nerve net in two-day-old planula detected using YL1/2 antibody. (A) Superficial view (optical plane on the basal part of the ectodermal epithelium). (B) Deeper view of the same specimen (optical plane crossing the larval endoderm and cavity). (C) Higher magnification view of the YL1/2 staining (in red) with Dapi counter-staining (in blue) showing the distribution and aspect of nerve cell bodies (white arrowheads) and neurites. Oral pole is on the top for all pictures. Scale bars: A-B, 50 μm; C, 10 μm.From: Multiple Sox genes are expressed in stem cells or in differentiating neuro-sensory cells in the hydrozoan Clytia hemisphaerica Muriel Jager, Eric Quéinnec, Hervé Le Guyader and Michaël Manuel. EvoDevo 2011, 2:12.
  • WB - Anti Tubulin Alpha Antibody, clone YL1/2  ABD12874
    Western blot analysis of C6 rat glioma whole cell lysate probed with Rat anti tubulin alpha antibody followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence
  • WB - Anti Tubulin Alpha Antibody, clone YL1/2  ABD12874
    HeLa whole cell lysate probed with Rat anti Tubulin Alpha:HRP
  • WB - Anti Tubulin Alpha Antibody, clone YL1/2  ABD12874
    Published customer image:Rat anti tubulin-αused as a loading control for cell lysates using western blotting.Image caption:Direct detection of the M42 polypeptide. (A) Validation of anti-M42 serum. Lysates from cells transfected with the indicated GFP polypeptides were analysed by SDS-PAGE and western blotting as labeled. (B) Detection of M42 from virus-infected cells. Lysates from cells infected with the indicated viruses at 10 h p.i. were analysed by SDS-PAGE and western blotting as labeled. The same membrane was probed with mouse anti-M2 14C2 and rabbit anti-M42 using different colour secondary antisera; individual grey scale and colour merged images are shown.From: Wise HM, Hutchinson EC, Jagger BW, Stuart AD, Kang ZH, et al. Identification of a Novel Splice Variant Form of the Influenza A Virus M2 Ion Channel with an Antigenically Distinct Ectodomain. PLoS Pathog 8: e1002998.
  • IF - Anti Tubulin Alpha Antibody, clone YL1/2  ABD12874
    Published customer image:Rat anti tubulin-α antibody, clone YL1/2 used for the detection of cytoskeleal elements in drosophila embryos by immunofluorescence.Image caption:Spindle abnormalities in embryos derived from imp-α2D14/imp-βKetRE34 and imp-α2D14/imp-βc02473; NLSB-/+ females. (A–D) Wild-type and mutant embryos stained for α-tubulin and DNA . (A) Mitotic spindles in wild-type embryos at metaphase and anaphase. (B, C) Categories of spindle abnormalities found in embryos derived from (B) imp-α2D14/imp-βKetRE34 and (C) imp-α2D14/imp-βc02743; NLSB-/+ females. (D) Formation of aster networks found in both genotypes. Scale bar: 10 μm. (E) Frequency of spindle defects in embryos from both types of mutant females. Female genotypes are displayed at the upper right corner. At least 200 spindles were scored for both genotypes.From: Virágh E, Gorjánácz M, Török I, Eichhorn T, Kallakuri S, Szlanka T, Kiss I, Mechler BM. Specific Cooperation Between Imp-α2 and Imp-β/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions. G3 . 2012 Jan;2(1):1-14.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Yeast
Host Rat
Clonality Monoclonal
Isotype IgG2a
Clone Names YL1/2
Additional Information
Other Species Ashbya,Birds,Echinoderm,H,M,Dog,Rat,Pig,D,Plants,Amphibia,Saccharomyces,Pleurobrachia,Caenorhabditis,Dictyostelium discoideum,X,Pig-tailed macaque,Clytia sp.,Arabidopsis,Strongylocentrotus purpuratus,Dendraster excentricus,Trypanosoma brucei,Potorous tridactylis,B,Nephrotoma suturalis no
Hemicentrotus pulcherrimus,Potato,Bombyx mori,Rhodnius prolixus
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Yeast tubulin
Shelf Life 18 months from date of despatch.
Target/Specificity Rat anti Tubulin alpha antibody, clone YL1/2 recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin) (Wehlandet al.1983). The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin).The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin (Burns 1987).This product is routinely tested in ELISA on Tubulin.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Tubulin Alpha Antibody, clone YL1/2 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12874
(40 western blots)
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