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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti Human CD126 (Il-6R) Antibody, clone B-R6    

Anti Human CD126 (Il-6R) Antibody, clone B-R6

Mouse Anti-Human Monoclonal Antibody

     
  • IF - Anti Human CD126 (Il-6R) Antibody, clone B-R6  ABD12887
    Published customer image:Mouse anti Human CD126 antibody, clone B-R6 used for the evaluation of IL6Rα expression on human muscle tissue by immunofluorescence.Image caption:Control Images: : Immunofluorescent stain of 7 µm muscle cross-sections for IL-6Rα (green - streptavidin-FITC), nuclei and the secondary antibody used for Pax7 (secondary only - Alexa 594) acquired using the 20× objective. Arrows denote nuclei that co-localize with IL-6Rα and do not show any non-specific secondary binding of the Alexa 594, or any Alexa 594 interaction with the IL-6Rα antibody. : Immunofluorescent stain of 7 μm muscle cross-sections for Pax7 (red - Alexa 594), nuclei and the secondary antibody used for IL-6 (secondary - tertiary only: immunoglobulin biotinylated secondary antibody + streptavidin-FITC) acquired using the 20× objective. Arrows denote nuclei that co-localize with Pax7 and do not show any non-specific secondary binding of the secondary antibodies , or any FITC interaction with the Pax7 antibody. : Immunofluorescent stain of 7 μm muscle cross-sections for IL-6 (green - streptavidin FITC), nuclei and the secondary antibody used for Pax7 (secondary only - Alexa 594) acquired using the 20× objective. Arrows denote nuclei that co-localize with IL-6 and do not show any non-specific secondary binding of the Alexa 594, or any Alexa 594 interaction with the IL-6 antibody. : Immunofluorescent stain of 7 μm muscle cross-sections for PCNA (red - Texas Red), nuclei and the secondary antibody used for Pax7 (secondary only - Alexa 488) acquired using the 20× objective. Arrows denote nuclei that co-localize with PCNA and do not show any non-specific secondary binding of the Alexa 488, or any Alexa 488 interaction with the PCNA antibody. : Immunofluorescent stain of 7 μm muscle cross-sections for Pax7 (green - Alexa 488), nuclei and the secondary antibody used for PCNA (secondary only: Texas Red) acquired using the 20× objective. Arrows denote nuclei that co-localize with Pax7 and do not show any non-specific secondary binding of the secondary antibody (Texas Red), or any Texas Red interaction with the Pax7 antibody. : Immunofluorescent stain of 7 μm muscle cross-sections for phosphorylated STAT3 (p-STAT3) (red - Texas Red), nuclei and the secondary antibody used for Pax7 (secondary only - Alexa 488) acquired using the 20× objective. Arrows denote nuclei that co-localize with p-STAT3 and do not show any non-specific secondary binding of the Alexa 488, or any Alexa 488 interaction with the PCNA antibody.From: McKay BR, De Lisio M, Johnston APW, O'Reilly CE, Phillips SM, Tarnopolsky MA, et al. Association of Interleukin-6 Signalling with the Muscle Stem Cell Response Following Muscle-Lengthening Contractions in Humans.PLoS ONE 4(6): e6027.
  • IF - Anti Human CD126 (Il-6R) Antibody, clone B-R6  ABD12887
    Published customer image:Mouse anti Human CD126 antibody, clone B-R6 used for the evaluation of IL6Rα expression on human muscle tissue by immunofluorescence.Image caption:Circulating IL-6 and muscle IL-6 mRNA and IL-6 receptor response muscle lengthening contractions . Average serum interleukin-6 (IL-6) concentration. Time 0 hr corresponds to pre-intervention values; all other time-points correspond to post-intervention time . Relative IL-6 mRNA expression, expressed as fold-change from 0 hr. Pearson correlation of serum IL-6 concentration versus muscle IL-6 mRNA , correlation is representative of the individual data points and presented as mean values (●)±SD (error bars). Relative IL-6 receptor (IL-6Rα) mRNA expression, expressed as fold-change from 0 hr. Immunofluorescent image (40×) of a muscle cross-section triple-stained for Pax7 , IL-6Rα and nuclei (DAPI?=?blue); IL-6Rα staining is apparent on the sarcolemma and satellite cell membrane. White arrow denotes one of the Pax7+ nuclei which co-localized with IL-6Rα (scale bar?=?100 μm). Values are reported as mean±S.E.M. Mean values represent the mean for all 8 subjects per time-point (8 samples per time-point, 40 samples total). *p<0.05 vs. 0 hr.From: McKay BR, De Lisio M, Johnston APW, O'Reilly CE, Phillips SM, Tarnopolsky MA, et al. Association of Interleukin-6 Signalling with the Muscle Stem Cell Response Following Muscle-Lengthening Contractions in Humans.PLoS ONE 4(6): e6027.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-F, IF, FC, E, IP
Primary Accession P08887
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names B-R6
Calculated MW 51548 Da
Additional Information
Purification Purified IgG prepared by ion exchange chromatography
Immunogen CHO cells transfected with cDNA of the IL-6 receptor.
Shelf Life 18 months from date of despatch.
Gene ID 3570
Other Names Interleukin-6 receptor subunit alpha, IL-6 receptor subunit alpha, IL-6R subunit alpha, IL-6R-alpha, IL-6RA, IL-6R 1, Membrane glycoprotein 80, gp80, CD126, IL6R
Target/Specificity Mouse anti Human CD126 antibody, clone B-R6 recognizes human Interleukin-6 receptor subunit alpha, also known as CD126, IL-6R or Membrane glycoprotein 80. Human CD126 is a 468 amino acid single pass type I transmembrane glycoprotein bearing a single Ig-like C2 type domain, two fibronectin type III domains and a single WSXWS motif which may be involved in receptor avtivation (Daqilet al.2012).Mouse anti Human CD126 antibody, clone B-R6 inhibits IL-6 mediated proliferation of XG-1 cells (Luet al.1995) and partially blocks binding of IL-6 to its receptor (Fourcinet al.1994), clone B-R6 recognizes both the soluble and membranous human CD126Removal of Sodium azide is recommended prior to use in functional assays. The use of EQU003 for this purpose.
Preservative & Stabilisers 0.09%Sodium Azide
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Human CD126 (Il-6R) Antibody, clone B-R6 is for research use only and not for use in diagnostic or therapeutic procedures.
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