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>   home   >   Products   >   Primary Antibodies   >   Anti Bovine CD1w2 Antibody, clone CC14    

Anti Bovine CD1w2 Antibody, clone CC14

Mouse Anti-Bovine Monoclonal Antibody

  • FC - Anti Bovine CD1w2 Antibody, clone CC14  ABD12889
    Published customer image:Mouse anti Bovine CD1w2 antibody, clone CC14 used for the evaluation of CD1w2 expression on bovine monocyte derived dendritic cells by flow cytometry.Image caption:Surface expression of MHCII (A), CD86 (B), CD1b (C) and CD40 (D) on cultured cells and fresh monocytes after exposure to crude polar and apolar lipid fractions. A single point represents the median fluorescence intensity of the specific stain after subtraction of an isotype control (ΔMFI) for each of 7 animals tested; ** p?<?0.01; *** p?<?0.001.From: Pirson C, Jones GJ, Steinbach S, Besra GS, Vordermeier HM. Differential effects of Mycobacterium bovis--derived polar and apolar lipid fractions on bovine innate immune cells.Vet Res. 2012 Jun 27;43:54.
  • FC - Anti Bovine CD1w2 Antibody, clone CC14  ABD12889
    Published customer image:Mouse anti Bovine CD1w2 antibody, clone CC14 used for the evaluation of CD1w2 expression on bovine myeloid cell subtypes by flow cytometry.Image caption:Phenotypic profiles of bovine myeloid cell sub-populations. Live gated PBMC were assessed for expression of CD16 and CD14 and a panel of molecules associated with antigen presentation, co-stimulation or specific cell lineages (see Table 1) by 3 colour flow cytometry. PBMC were stained with primary mAb to the specific molecules indicated and then with an isotype-specific PE conjugated secondary, followed by CD16 and CD14 conjugated to FITC or Alexa Fluor 647 respectively. Live, single PBMC were gated based on the expression of CD16 and CD14 as detailed in Figures 1 and 2C. Histograms show the levels of expression of selected markers in the cell populations studied, CD14 - CD16 ++ , CD14 + CD16 + and CD14 + CD16 low/- compared to PBMC . Data shown are for one representative animal of four animals.From: Corripio-Miyar Y, Hope J, McInnes CJ, Wattegedera SR, Jensen K, Pang Y, Entrican G, Glass EJ. Phenotypic and functional analysis of monocyte populations in cattle peripheral blood identifies a subset with high endocytic and allogeneic T-cell stimulatory capacity.Vet Res. 2015 Sep 25;46(1):112.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Reactivity Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names CC14
Additional Information
Other Species Ga,Sh
Purification Purified IgG prepared by affinity chromatography on Protein G
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti Bovine CD1w2 antibody, clone CC14 recognizes the CD1w2 cell surface antigen in bovine, sheep and goat.Mouse anti Bovine CD1w2 antibody, clone CC14 immunoprecipitates two protein bands of ~12 kDa and ~46 kDa. In immunohistology the reagent stains cortical thymocytes and a minority of medullary thymocytes, with a pattern similar to antibodies of the CD1b cluster in humans.
Preservative & Stabilisers 0.09%Sodium Azide
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Bovine CD1w2 Antibody, clone CC14 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12889
(40 western blots)
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