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>   home   >   Products   >   Primary Antibodies   >   Anti Bovine CD4 Antibody, clone CC30    

Anti Bovine CD4 Antibody, clone CC30

Mouse Anti-Bovine Monoclonal Antibody

     
  • IHC-P - Anti Bovine CD4 Antibody, clone CC30  ABD12892
    Published customer image:Mouse anti Bovine CD4 antibody, clone CC30 used for the detection of CD4+ve helper T cells by immunohistochemistry on formalin fixed, paraffin embedded placental sections.Image caption:Examples of different immune cell infiltration in placentomes from N. caninum-inoculated animals. (a) Severe infiltration of macrophages in the base of maternal caruncle of a N. caninum inoculated cow culled 14 dpi. Counterstained with haematoxylin. Original magnification 100×. (b) Mild aggregate of T helper cells (CD4+) surrounding a blood vessel in maternal caruncle of a placentome collected from an inoculated cow culled 28 dpi. Counterstained with haematoxylin. Original magnification 200×. (c) Minimal infiltration of cytotoxic T-cells (CD8+) in a necrotic focus in maternal caruncle of a N. caninum challenged cow culled 28 dpi. Counterstained with haematoxylin. Original magnification 200×. (d) Mild aggregate of γδ T-cells in the base of maternal caruncle of an inoculated cow culled 14 dpi. Counterstained with haematoxylin. Original magnification 200×. (e) Rare presence of NK-cells in connective tissue in the maternal caruncle surrounding necrotic and mineralized foetal villi, observed in a placenta of an inoculated cow culled 28 dpi. Counterstained with haematoxylin. Original magnification 200×. (f) Rare presence of CD79αcy+ cells aligned with endometrial epithelial cells in the maternal caruncle and in some foetal villi, observed in the placentome of a N. caninum inoculated cow culled 28 dpi. Original magnification 400×. Counterstained with haematoxylin.From: Cantón GJ, Katzer F, Benavides-Silván J, Maley SW, Palarea-Albaladejo J, Pang Y, Smith S, Bartley PM, Rocchi M, Innes EA, Chianini F.Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation.Vet Res. 2013 Jul 22;44:60.
  • IHC - Anti Bovine CD4 Antibody, clone CC30  ABD12892
    Published customer image:Mouse anti Bovine CD4 antibody, clone CC30 used to demonstrate the presence of T helper cells in ovine placenta during gestation by immunohistochemistry on cryostat sections.Image caption:Comparison of the immunohistochemical labelling of T inflammatory cells and parasite antigen in the placenta. This panel compares the distribution and frequency of T lymphocytes (CD3, CD4 and CD8 antigens) and parasite antigen immunohistochemically labelled in samples from the three groups. Pictures showing the labelling of T cells (first three rows) were taken at 75× and those of the parasite antigen (last row) were taken at 150×. F: foetal mesenchyme, M: maternal endometrial stalk.From: Arranz-Solís D, Benavides J, Regidor-Cerrillo J, Horcajo P, Castaño P, Del Carmen Ferreras M, Jiménez-Pelayo L, Collantes-Fernández E, Ferre I, Hemphill A, Pérez V, Ortega-Mora LM.Systemic and local immune responses in sheep after Neospora caninum experimental infection at early, mid and late gestation.Vet Res. 2016 Jan 6;47(1):2.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IHC-F, FC, IP
Reactivity Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names CC30
Additional Information
Other Species Bison
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Bovine thymocytes.
Shelf Life 18 months from date of despatch.
Target/Specificity Mouse anti Bovine CD4 antibody, clone CC30 recognizes a ~50 kDa transmembrane molecule considered to be the bovine homologue of human CD4. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected.Mouse anti Bovine CD4, clone CC30 has successfully been used for immunohostochemical localization of CD4 on paraffin embedded material using zinc salt fixation (Cantónet al.2013). Additionally, clone CC30 has been reported as being suitable for use on formal dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Gutierrezet al.1999).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Bovine CD4 Antibody, clone CC30 is for research use only and not for use in diagnostic or therapeutic procedures.
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