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>   home   >   Products   >   Primary Antibodies   >   Anti Bovine CD8 Antibody, clone CC63    

Anti Bovine CD8 Antibody, clone CC63

Mouse Anti-Bovine Monoclonal Antibody

  • FC - Anti Bovine CD8 Antibody, clone CC63  ABD12894
    Bovine lymph node stained with Mouse anti Bovine CD8 10x. Image courtesy of Dr. J.B. Silvan, Moredun Research Institute, Roslin, UK
  • IF - Anti Bovine CD8 Antibody, clone CC63  ABD12894
    Published customer image:Mouse anti Bovine CD8 antibody, clone CC63 used for the identification of CD8 expressing cells inthe nasopharynx by immunofluorescence. Image caption:Nasopharyngeal primary infection sites of vaccinated cattle are infiltrated by CD8+/CD3- (presumptive NK) cells at 24 hpi.Multichannel immunofluorescent technique. A). FMDV VP1 protein within cytokeratin+ cells in epithelial crypt of the nasopharyngeal mucosa of non-vaccinated steer at 24 hpi (animal number 3). CD8+ / CD3+ double-positive CTLs are present in the subepithelial compartment amongst larger populations of CD8-/CD3+ T-lymphocytes. 20x magnification, scale bar 50μm. B) 40x magnification of region identified in (A), demonstrating consistent co-localization of CD8 with CD3 . Scale bars 25μm. B) Select channels of image shown in (B). CD3+ cells include single-positive (T-lymphocytes) or CD8+/CD3+ double-positive . CD8 is exclusively detected in combination with CD3 . Scale bars 25 μm. D) FMDV VP1 protein co-localize with cytokeratin in a focal surface erosion within follicle-associated epithelium of nasopharyngeal mucosa of vaccinated steer at 24 hpi (animal number 11). A distinct population of cells defined as presumptive NK-cells based on a CD8+ /CD3- phenotype is present in submucosal and epithelial compartments surrounding the focus of infection. A smaller population of CTLs (CD8+/CD3+) is present amongst abundant non-CTL T-lymphocytes (CD8-/CD3+). 20x magnification, scale bar 50μm. E) Higher magnification of region identified in showing CD8+/CD3- cells representing presumptive NK cells in close proximity of FMDV infected focus. 40x magnification, scale bars 25 μm F) Individual channels of image shown in (E) demonstrating inconsistent co-localization of CD8 and CD3 . Scale bar 25 μm.From: Stenfeldt C, Eschbaumer M, Pacheco JM, Rekant SI, Rodriguez LL, Arzt J Pathogenesis of Primary Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Vaccinated and Non-Vaccinated Cattle.PLoS ONE 10: e0143666.
  • FC - Anti Bovine CD8 Antibody, clone CC63  ABD12894
    Bovine lymph node stained with Mouse anti Bovine CD8 40x. Image courtesy of Dr. J.B. Silvan, Moredun Research Institute, Roslin, UK
  • FC - Anti Bovine CD8 Antibody, clone CC63  ABD12894
    Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD8: FITC
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P31783
Reactivity Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names CC63
Calculated MW 26417 Da
Additional Information
Other Species Sh,Ga
Purification Purified IgG prepared by affinity chromatography on Protein G
Shelf Life 18 months from date of despatch.
Gene ID 281060
Other Names T-cell surface glycoprotein CD8 alpha chain, CD8a, CD8A
Target/Specificity Mouse anti Bovine CD8 antibody, clone CC63 reacts with the bovine CD8 antigen expressed by a subset of T lymphocytes. The antibody precipitates molecules of 34kD and 38kD under reducing conditions. Clone CC63 has been reported as being suitable for use on formalin dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Gutierrezet al.1999).
Preservative & Stabilisers 0.09% Sodium Azide, 1% Bovine Serum Albumin
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Bovine CD8 Antibody, clone CC63 is for research use only and not for use in diagnostic or therapeutic procedures.
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Cat# ABD12894
(40 western blots)
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