Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   Anti Myelin Proteolipid Protein Antibody, clone plpc1    

Anti Myelin Proteolipid Protein Antibody, clone plpc1

Mouse Anti-Bovine Monoclonal Antibody

     
  • WB - Anti Myelin Proteolipid Protein Antibody, clone plpc1  ABD12896
    Published customer image:Mouse anti myelin proteolipid protein antibody, clone plpc1 used for detection of myelin proteolipid protein in human and porcine brain lysates by western blottingImage caption:Validation of monomer and oligomer preference of aSN interacting proteins. Proteins pulled down by monomer aSN (M), oligomer aSN (O), and buffer control (B) from porcine (A-F) and human (G-I) brain extracts were analyzed by immunoblotting using antibodies against antigens selected among the monomer and oligomer binding proteins. Monomer binding antigens were myelin proteolipid protein and Abl interactor 1 and oligomer binding proteins were glial fibrillary acidic protein , glutamate decarboxylase 2 , and synapsin 1 . VAMP-2 was tested because it has been reported to bind aSN, although it was not detected in our proteomic analysis. One representative of three experiments is presented for porcine aSN binding proteins (A, C, E), and the quantification of the three experiments is presented in panels B, D, F. The quantification of bands was performed after subtracting the non-specific signal in the buffer control from the specific bands in monomer and oligomer samples. Bars represent mean ratio between monomer and oligomer ± S.D. of the three replicates. The values for binding to monomer and oligomer were compared by Student’s t-test and the resulting p-values are listed above the bars. * Indicates that the band intensity from oligomer did not differ significantly from background making quantifications impracticable. In order to ensure that the interaction were not due to species differences between human and porcine proteins we conducted validations in human brain extracts. One representative of two experiments is presented for each validated protein. The validation for both porcine and human of mPLP, Abl1, Syn1 and VAMP-2 was conducted in the LP2 fraction enriched in synaptic vesicle and the validation to GFAP and GAD2 in the LS1 fraction of synaptosomal lysate.From: Betzer C, Movius AJ, Shi M, Gai W-P, Zhang J, et al. Identification of Synaptosomal Proteins Binding to Monomeric and Oligomeric a-Synuclein. PLoS ONE 10(2): e0116473.
  • WB - Anti Myelin Proteolipid Protein Antibody, clone plpc1  ABD12896
    Published customer image:Mouse anti myelin proteolipid protein antibody, clone plpc1 used for detection of myelin proteolipid protein in brain lysates by western blottingImage caption:Presence of aSN binding proteins chosen for validation in synaptosomal fractions. 30 µg of each of the fractions synaptosomal membranes LP1, synaptosomal lysate , synaptic vesicles and synaptosomal cytosol were immunoblottet, and analyzed for the presence of myelin Proteolipid protein , Abl interactor 1 , Glial fibrillary acidic protein , Glutamic acid decarboxylase 2 , Synapsin 1 , and Vesicle associated membrane protein 2 .From: Betzer C, Movius AJ, Shi M, Gai W-P, Zhang J, et al. Identification of Synaptosomal Proteins Binding to Monomeric and Oligomeric a-Synuclein. PLoS ONE 10(2): e0116473.
  • IHC - Anti Myelin Proteolipid Protein Antibody, clone plpc1  ABD12896
    Published customer image:Mouse anti myelin proteolipid protein antibody, clone plpc1 used for detection of proteolipid protein in formalin fixed, paraffin embedded multiple sclerosis brain tissueImage caption:Preactive lesions are composed of clusters of HLA-DR-positive microglia. In NAWM, i.e., in the absence of apparent myelin loss (A, E: proteolipid protein) preactive lesions are defined as circumscribed nodules of activated microglia expressing HLA-DR (B, C) and CD68 (D). Preactive lesions are predominantly observed in blocks containing active lesions (E: proteolipid protein; F, G: HLA-DR). Figure1G represents a magnification of the outlined square in (F). In some cases microglial nodules are surrounded by a halo devoid of microglia (H: HLA-DR). Original magnifications: A, B: 20×; C, D: 40×; E, F: 4×; G, H: 40×.From: van Horssen J, Singh S, van der Pol S, Kipp M, Lim JL, Peferoen L, Gerritsen W, Kooi EJ, Witte ME, Geurts JJ, de Vries HE, Peferoen-Baert R, van den Elsen PJ, van der Valk P, Amor S. Clusters of activated microglia in normal-appearing white matter show signs of innate immune activation. J Neuroinflammation. 2012 Jul 2;9:156.
  • IHC-P - Anti Myelin Proteolipid Protein Antibody, clone plpc1  ABD12896
    Published customer image:Mouse anti myelin proteolipid protein antibody, clone plpc1 used for the detection of proteolipid protein on formalin fixed, paraffin embedded brain tissue.Image caption:NLs on MRI and tissue sections stained for PLP and iron. Examples Examples of NLs from tissue MS1 (A, B and C) identified on MRI magnitude (TE = 25.2 ms) and R2* images as well as color-coded PLP-staining and iron staining. Red arrows point towards NLs identified by MRI and confirmed to correspond to area of demyelination by the color-coded PLP staining. Blue arrows point towards NLs identified by the color-coded PLP staining and only retrospectively identified by MRI. Black arrows and black box point towards NLs identified by the color-coded PLP staining and not by MRI even upon a second retrospective image inspection. In the black box we include a large area of demyelination which goes entirely undetected by MRI. In the color-coded PLP staining of figure: green = complete WM demyelination, red = complete GM demyelination, yellow = areas of variably reduced myelin density.From:Yao B, Hametner S, van Gelderen P, Merkle H, Chen C, et al. 7 Tesla Magnetic Resonance Imaging to Detect Cortical Pathology in Multiple Sclerosis. PLoS ONE 9: e108863. doi:10.1371/journal.pone.0108863
  • IHC-P - Anti Myelin Proteolipid Protein Antibody, clone plpc1  ABD12896
    Published customer image:Mouse anti myelin proteolipid protein antibody, clone plpc1 used for the detection of proteolipid protein on formalin fixed, paraffin embedded brain tissue.Image caption:Iron loss in NLs. PLP myelin staining (B) and iron staining (C) on two consecutive slides of tissue MS2 disclose iron loss together with myelin loss in a NL (red arrows). Higher magnification (D, E, F, G) of PLP (D, F) and iron stainings (E, G). In NAGM (D, E), iron is present in oligodendrocytes (black arrow and inset in E) and myelin sheaths. In a NL (F, G), iron is removed from the demyelinated cortical parenchyma and present in perivascular macrophages and microglia (black arrow and inset in G).From:Yao B, Hametner S, van Gelderen P, Merkle H, Chen C, et al. 7 Tesla Magnetic Resonance Imaging to Detect Cortical Pathology in Multiple Sclerosis. PLoS ONE 9: e108863.doi:10.1371/journal.pone.0108863
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, FC
Primary Accession P04116
Reactivity Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names plpc1
Calculated MW 30015 Da
Additional Information
Other Species H
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen Synthetic peptide GRGTKF corresponding to C terminal region of myelin proteolipid protein.
Shelf Life 18 months from date of despatch.
Gene ID 281410
Other Names Myelin proteolipid protein, PLP, Lipophilin, PLP1, PLP
Target/Specificity Mouse anti myelin proteolipid protein antibody, clone plpc1 recognizes myelin proteolipid protein (PLP) in many mammalian species (Stoffelet al.1985). Clone plpc1 also recognizes the alternative PLP splice variant lacking part of the cytoplasmic domain (amino acids 117-151), known as DM20 (Simonset al.1987) .PLP encodes the major protein components of compact CNS myelin and mutations in the PLP gene can lead to severe dysmyelinating disease (Hudsonet al.1989).Mouse anti myelin proteolipid protein, clone plpc1 has proved a useful immunohistochemical tool for the study of central nervous system injury in patients with multiple sclerosis (Seewanet al.2011,Huizingaet al.2011)
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20℃ if preferred.
PrecautionsAnti Myelin Proteolipid Protein Antibody, clone plpc1 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to
info@abgent.com, and receive a free "I Love Antibodies" mug.
FeedBack
Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abgent.com.

Cat# ABD12896
Size:
Quantity:
Availability: Inquire
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Crown Flash17-30% Off
Terms & Conditions